Gibco® - HEPES
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HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent and is...
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent and is categorized as a "Good" buffer which derives from a set of buffers described by Dr. Norman Good and his colleagues in 1966 (Good et. al., Biochemistry 1966).
HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media.
HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media.
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Features of HEPES
"Good buffers" such as HEPES are attributed with the following characteristics:
- pKa values between 6.0 and 8.0
- High solubility
- Membrane impermeability
- Limited effect on biochemical reactions
- Very low visible and ultraviolet light absorbance
- Chemically and enzymatically stable
- Easy to prepare.
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