Tecan - HydroSpeed
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The expert plate washer for cells, beads and ELISAs in 96- and 384-well format
Cell Assays
Cell Protection™ extra gentle drop-wise dispensing to minimize cell detachment. Reduced turbulence via online optimization of dispense position. Typical cell recovery of =85% using tuneable aspiration settings.
Bead Assays
Fast bead settling thanks to a patent pending design using two magnets per well. High bead recovery rates (typically =90 %) via tuneable aspiration settings. Optional vacuum filtration for non-magnetic bead washing.
ELISAs
Anti-Clogging™ active prevention of needle blockage for reliable operation. Automated rinsing and soaking of wash head for easy handling. Easy X-change wash heads for straightforward ultrasonic cleaning.
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Features of HydroSpeed
Cell Protection™
Extra gentle cell washing with tuneable wash power Loosely adherent cell lines, such as P815 and HEK 293, are commonly used for a wide range of research applications, but are challenging to wash for typical plate washers.
Discover the difference with Tecan's HydroSpeed expert cell washer:
- Drop-wise dispense speed to avoid cell detachment
- Optimization of dispense position to minimize turbulence in the wells
- Tunable aspiration parameters, including needle position and vacuum level, to avoid cell loss
- Reduced costs by eliminating the need for a dedicated cell washing head
Anti-Clogging™
Reliable operation through active prevention of needle clogging Automated ELISA processing using a microplate washer is now a routine task for many laboratories, yet crystallization of wash buffer within the aspiration/dispense needles remains a common problem. The HydroSpeed's innovative Anti-Clogging function actively prevents needle clogging by automatically rinsing and soaking the wash head when the washer is idle for a user-defined period.
Highlights of Anti-Clogging function include:
- Automated start of rinse & soak steps of the wash head to avoid buffer crystallization
- Fail safe procedure in case user has forgotten to start daily maintenance program
- No downtime for cleaning of spills typically caused by needle blockage
- Easy X-change wash heads allows additional intense cleaning in an external ultrasonic bath
Patent pending design using dual magnets per well
Efficient bead settling and high bead recovery rates Multiplexed assays using magnetic beads are becoming increasingly popular for a variety of applications, but achieving good wash results without loosing beads remains a challenge. The HydroSpeed washer protects magnetic beads by settling them away from the needles during aspiration, achieving low residual volumes while maintaining high bead recovery rates.
Features include:
- Powerful rare-earth magnets to provide fast and efficient bead settling
- Two magnets per well to keep the beads out of the way during washing
- Adjustable vacuum level and aspiration position for optimized bead recovery
Ease of use
Integrated monitoring of liquid level in waste bottle
- Integrated pressure-based liquid level detection (p-LLD) system informs operator when waste bottle is full
- Patent pending p-LLD function avoids the risk of blockage common to swimmer-based LLD systems mounted in the waste bottle, increasing safety and eliminating additional cleaning steps
Patent-pending auto-adjust function for easy teaching of new plates
- Provides automated adjustment of the bottom aspiration position located inside the wells for optimized wash results
- Takes the hassle out of teaching plates with non-transparent wells typically used for reading of fluorescence or luminescence assays
- Handles different well geometries in 96- & 384-plate formats including flat-bottom, round-bottom and v-shaped wells
General Specifications
| Depth | 16.9 in |
| Height | 11.3 in |
| Width | 15.3 in |
| Power Requirements | 115 V / 60 Hz, |
| Peak Power | 300 VA |
| Weight | 33 lbs |
Additional Specifications
General
Number of disp. channels:
1–4
Wash head types:
96HT for high speed washing in 96-well format
384HT for high speed washing in 384-well format
96 indexing for flexible washing in 96- & 384-well formats
Wash volume, 96-well format:
50–3000 Μl in increments of 50 Μl
Wash volume, 384-well format:
10–1000 Μl in increments of 10 Μl
Performance
Wash times, 96-well format:
15 secs for 1-cycle wash inc. crosswise asp. step
(disp. vol: 300 Μl/well, disp. rate: 5, asp. rate: 5, 96HT head)
Wash times, 384-well format:
15 secs for 1-cycle wash inc. asp. step
(disp. vol: 100 Μl/well, disp. rate: 5, asp. rate: 5, 384HT head)
Dispense accuracy @ 300 Μl, 96-well format:
= 5 % across plate, gravimetric measurement*
Dispense accuracy @ 100 Μl, 384-well format:
= 5 % across plate, gravimetric measurement**
Dispense uniformity @ 300 Μl, 96-well format:
= 3 % across plate*
Dispense uniformity @ 100 Μl, 384-well format:
= 4 % across plate**
Residual volume, 96-well format:
< 2 Μl per well, crosswise asp.*
Residual volume, 384-well format:
= 2Μl per well, single asp.**
Vacuum range for vacuum filtration:
-50 to -300 mBar
Videos
Tecan HydroSpeed™