Molecular Devices - IMAP Kinase, Phosphatase, and Phosphodiesterase Assays
Manufactured by Molecular Devices
IMAP technology provides a non-radioactive, homogeneous assay for accurate determination of kinase, phosphatase, and phosphodiesterase activities.
Until recently, assays of kinase activity have been performed using radioactive isotopes or highly specific antibodies. In contrast, IMAPĀ® technology provides a non-radioactive, homogeneous assay applicable to a wide variety of kinases without regard for substrate peptide sequence. The assay is a simple "mix-and-read" procedure that allows accurate determination of kinase, phosphatase, and phosphodiesterase (PDE) activity.
Based on the specific, high-affinity interaction of trivalent metal-containing nanoparticles with phosphogroups, IMAP is a generic platform to assess kinase, phosphatase, and phosphodiesterase activity. An enzyme reaction is performed using fluorescently labeled substrate. Addition of the IMAP Binding System stops the enzyme reaction and specifically binds the phosphorylated substrates. Binding of the substrate to the beads can be detected using either FP or TR-FRET as a readout.
Based on the specific, high-affinity interaction of trivalent metal-containing nanoparticles with phosphogroups, IMAP is a generic platform to assess kinase, phosphatase, and phosphodiesterase activity. An enzyme reaction is performed using fluorescently labeled substrate. Addition of the IMAP Binding System stops the enzyme reaction and specifically binds the phosphorylated substrates. Binding of the substrate to the beads can be detected using either FP or TR-FRET as a readout.
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Features of IMAP Kinase, Phosphatase, and Phosphodiesterase Assays
- IMAP provides a complete assay system for screening kinases, phosphatases, and phosphodiesterases.
- Because IMAP assays are not antibody-based, they are generic and can be used for any kinase, phosphatase, or phosphodiesterase.
- Robust fluorescence signal gives reliable results with good Z factors.
- IMAP assays are homogeneous and amenable to miniaturization for greater cost savings.
- IMAP assays are available in both FP and TR-FRET detection modes to meet users' screening needs.
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