Molecular Devices - QBT Fatty Acid Transporter Assay

Manufactured by  Molecular Devices
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The QBT™ Fatty Acid Uptake Assay is a single-step, homogeneous, fluorescent assay for monitoring the activity of fatty acid transport proteins.

Conventional protocols for monitoring fatty acid uptake using radioactivity often require cell lysis and processing at very low temperature, making them expensive, slow and unsuitable for high throughput screening. Other fluorescence-based protocols generally require the use of low throughput fluorescence activated cell sorter (FACS) instrumentation, or require cell washing, which can severely compromise the integrity of the fragile adipocytes used in these assays. 
The QBT™ Fatty Acid Uptake Assay is a homogeneous assay amenable to high-throughput screening. It uses a BODIPY® -dodecanoic acid fluorescent fatty acid analog coupled with Molecular Devices proprietary quench technology. The BODIPY label provides an ideal long chain fatty acid analog that behaves much like natural fatty acids and is a known substrate for fatty acid transporters. The elimination of radioactive compounds results in easier reagent handling, reduced disposal costs and eliminates safety risks associated with radiolabel assays.
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Features of QBT Fatty Acid Transporter Assay

Benefits of the QBT™ Fatty Acid Uptake Assay Kit 

  • Replaces cumbersome and time-consuming radioactive assays, reducing disposal costs and eliminating safety risks associated with radioactive assays. 
  • One-step, homogeneous assay format enables high-throughput screening. 
  • Fluorescent assay with proprietary quench technology allows users to monitor real-time uptake kinetics in live cells.
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