GE Healthcare - Superdex 200 Increase 10/300 GL

Manufactured by  GE Healthcare
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Superdex 200 Increase 10/300 GL is a versatile, prepacked column

Superdex 200 Increase 10/300 GL is a versatile, prepacked column for size exclusion chromatography in small-scale (mg) preparative purification as well as for characterization and analysis of proteins with molecular weights between 10 000 and 600 000, such as antibodies.  
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Features of Superdex 200 Increase 10/300 GL
  • Increased resolution vs Superdex 200.    
  • Reduced runtime vs Superdex 200 for quicker results.    
  • Versatile use for both preparative and analytical purposes.    

Superdex 200 Increase is the next generation of size exclusion chromatography (gel filtration) media offering versatility and higher resolution within a shorter time frame compared to Superdex 200. The Superdex 200 Increase 10/300 GL column is well suited for small-scale (25 – 500 µl) preparative purification such as the polishing step in a purification procedure, where mg amounts of highly size-homogenous target proteins can be achieved. It is also well suited for characterization of proteins, such as aggregate analysis of antibodies, size homogeneity analysis of membrane proteins, or protein-protein interactions, where high-quality data can be achieved.

General Specifications
pH Range3 to 12
Additional Specifications

Bed Dimensions: 10 × 300-310 mm       
Bed Volume: Approximately 24 ml       
Complete Packsize : 1       
Flow Rate: 48 000 m   
Pressure Max. [Over the Packed Bed During Operation] : 48 000 m 
Recommended Flow Rate: 0.75 ml/min  
Sample Volume: 25 µl – 500 µl  
Storage Conditions : 4° to 30° C, 20% ethanol  
Theoretical Plates: > 48 000 m

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