BERTHOLD TECHNOLOGIES - Mithras2 LB 943 Monochromator Multimode Reader

Manufactured by  BERTHOLD TECHNOLOGIES
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Mithras² LB 943 is a high-end microplate multimode reader based on monochromator technology with excellent performance.

Mithras² LB 943 is a high-end microplate multimode reader based on monochromator technology with excellent performance. Renowned for its sensitivity and robustness especially in luminescence and BRET measurements the reader supports all important reading technologies.     
     
In contrast to the usual kind of multi-technology instruments the Mithras multimode microplate reader has been designed with a proprietary optical system consisting of separate paths for different reading technologies (mDOPS), thus offering performances known from dedicated instruments. As each technology has its own demands on the optical system only separated light paths ensure that the needs for high sensitivity and a wide dynamic range are met with each technology.
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Features of Mithras2 LB 943 Monochromator Multimode Reader
Double Monochromators      Mithras² LB 943 employs two double monochromators for excitation and emission respectively. The monochromators are predominately meant to provide entire wavelength flexibility in absorbance and fluorescence measurents, yet they can be used for Time-Resolved Fluorescence and Luminescence measurements as well. While a low f number and a high blocking ensure exceptional performance variable bandwidths and increments of 1 nm illustrate the versatility of the monochromators.            RFID-coded Filters      For applications requiring the high light transmission of filters, e.g. BRET and Fluorescence Polarization, the instrument can be equipped with filters. RFID-coding of the filters enable and secure their error-free usage.            Built-in Reagent Injectors      Up to 4 reagent injectors can be set up to dispense multiple activators or detection reagents at any time and with user-adjustable volumes during the measurement.  Berthold Technologies´ injectors are based on the proprietary JET Injection technology. Superior accuracy and precision is combined with excellent mixing performance. Variable injections speeds and "friendly" material enable even injection of live cells (e.g. in       Aequorin based GPCR screening).            The injector tips can be located right in reading position enabling the measurement of very fast labels and reactions, e.g. Acridinium esters and Calcium flux, and to inject activators into a running kinetic.            Waste Pump      Excess fluids from priming and washing procedures are safely discarded via an automatic waste pump.            Temperature Control      A temperature controlled incubation area ensures stable conditions for the micro-plate whenever temperature sensitive enzymes or cells are in use. Condensation of lidded micro-plates is reduced.            Automatic Plate Height Adjustment (Z Optimization)      The plate height adjustment module detects the height of the plate used and automatically adjusts the measurement optics for it. There is no need any more for users to perform time-consuming Z-scans. Besides optimizing the focus of the excitation beam it also ensures there will be no crosstalk from adjacent wells.
General Specifications
Depth 550 mm
Spectral Range200 to 1000 nm
Height500 mm
Width 480 mm
Detection Methodlow noise photomultiplier tube
Shaking Pattern3 modes
FiltersRFID coded
Sample Formats6 – 1536 well plates
Weight48 kg
Temperature Control 1
Additional Specifications

Detection Devices:      
2 low-noise photomultiplier tube operated in single photon counting mode, spectral range up to 850 nm     
ultra-low-noise photomultiplier tube operated in single photon counting mode, spectral range up to 650 nm (HiSens position)     
photo diode, spectral range 200 – 1000 nm     
Excitation Sources:     
halogen lamp     
Xenon flash lamp     
laser, 680 nm (Alpha Technology option)     
Monochromator:     
f number: 2.7 (high light transmission)     
variable bandwidth: 6 - 22 nm     
increment: 1 nm     
Blocking:      
10-6     
Filters RFID coded     
Sample/Plate Formats:     
6 – 1536 well plates     
Terasaki plates     
Petri dishes     
filter membranes     
Temperature Control:     
up to 45 °C plate incubation area     
Shaking: 3 modes, 3 velocities     
Scanning: up to 10,000 points per well     
Injection:      
up to 4 JET injectors     
variable volume 10 – 100 µL     
variable speed     
tips can be chosen to be located in 8 positions     
Kinetics Operation:     
minimum resolution 0.05 sec     
maximum duration up to 7 days     
Automation:     
robot integration     
[u]Performance[/u]      
Luminescence:     
900 %     
Fluorescence Polar.:     
6 decades (PMT)     
4 decades (photo diode)     
Injectors:     
accuracy better 98 % over entire volume range     
precision better 98 % over entire volume range     
[u]Miscellaneous: [/u]     
Dimensions:     
480 x 500 x 550 mm (w x h x d) incl. injectors     
Weight: 48 kg     
OS: Win XP, Win Vista, Win 7     
Regulations: CE, UL, CSA

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