Applied Scientific Instrumentation - diSPIM PR

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Dual Inverted Selective Plane Illumination Microscopy

Dual Inverted Selective Plane Illumination Microscopy.docx   
   
This microscope has multicolor capability and has been tested successfully on cells cultured on cover slips, cells embedded in collagen gels and nematode and zebrafish embryos.
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Features of diSPIM PR
  • Generate 3d volumes with isotropic resolution (330) nm in all direction).       * Axial resolution is ~2x better than confocal or spinning disk systems.       * Acquisition rates up to 200 images per second or 2-5 volumes per second (roughly 10 times faster than spinning disk microscopes).       * Achieve a ~7-10 fold reduction in photobleaching.       * Use conventional mounting and glass cover slips.
General Specifications
Microscope TypeInverted

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