Sigma-Aldrich - Ascentis Express

Manufactured by  Sigma-Aldrich
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Supelco® Introduces Ascentis® Express HILIC HPLC Columns for High-Speed and High-Efficiency Separations

Supelco, a division of Sigma-Aldrich, has expanded the Ascentis Express product line, a family of innovative high-purity liquid chromatography (HPLC) columns designed to provide the analysis speed and resolving power of sub-2-micron columns with a 50% reduction in backpressure. Ascentis Express HILIC can perform high-speed and high-resolution analyses on highly polar compounds.

The enabling technology for Ascentis Express is the Fused-Core™ particle. These high-purity silica particles measure 2.7 microns overall with a 0.5 micron-thick porous shell. Relative to columns using conventional sub-2-micron particles, Ascentis Express columns generate comparable efficiencies but with half the backpressure.
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Features of Ascentis Express

There are five key user benefits with the Ascentis Express HPLC Columns:

1. Hyper-Fast Separations

  • Designed for high flow rates and sample throughput, especially LC-MS* Half of the backpressure of sub-2-µm particles

    2. High-Definition Resolution* Twice the efficiency of 3 µm particles* Longer columns permit doubling the plates over sub-2-µm particles* Shortest analyte diffusion path

    3. Super-Sensitive* Sharper peaks provide better Signal:Noise

    4. Super-Rugged* Narrowest particle size distribution* 2µm porosity frits like most rugged conventional columns

    5. A Family of selectivities that rivals the competition* C18, RP-Amide, Phenyl-Hexyl, C8, HILIC* Match competitor selectivitiy and performance at lower pressure

General Specifications

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Additional Specifications

Ascentis® Express is available in the following:

Ascentis Express C18 HPLC Columns
Ascentis Express RP-Amide HPLC Columns
Ascentis Express Phenyl-Hexyl HPLC Columns
Ascentis Express C8 HPLC Columns
Ascentis Express HILIC HPLC Columns

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    HPLC Column Fundamentals (28 min) provides an introduction to how solutes can interact with columns containing porous particles and stationary phases to result in separation. The efficiency, retention and selectivity terms in the ...

    HPLC Column Fundamentals

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