PerkinElmer - 9600

Manufactured by  PerkinElmer
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The GeneAmp PCR System 9600 automates the Polymerase Chain Reaction (PCR) technique for amplifying DNA. The Polymerase...

The GeneAmp PCR System 9600 automates the Polymerase Chain Reaction (PCR) technique for amplifying DNA. The Polymerase Chain Reaction (PCR) technique is conceptually a very simple method for amplifying nucleic acids. It mimics the natural DNA replication process in that the number of DNA molecules generated by the Polymerase Chain Reaction doubles after each cycle. In the PCR technique, the typical cycle consists of three steps:

1) Denaturing the template DNA by heating it to a high temperature (94 degrees C to 95 degrees C), thereby producing two single strands of DNA.

2) Annealing of the target-specific primers to the two separated DNA strands by cooling the reaction mixture to a lower temperature (37 degrees C to 65 degrees C).

3) Extending the annealed primers with a DNA polymerase by warming the reaction mixture to an intermediate temperature (72 degrees C).
The anneal and extend steps can be combined into a single setpoint, called two-temperature PCR.

The original DNA segment can be either a small, discrete molecule whose sequence is already known or a part of a much larger molecule in a complex mixture, such as a chromosome fragment.

The product of the reaction will be discrete double-stranded DNA molecules whose termini will be determined by the set of primers used. Because the copy number of the target DNA doubles after each cycle, amplification through 25 cycles would theoretically yield a 33 million fold increase of specific product.
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Features of 9600
  • Reproducibility of cycle times
  • Uniformity of PCR yields for samples
  • Sample tray accommodating up to 96 samples in an 8 x 12 format
  • Thin-walled MicroAmp Reaction Tubes allowing efficient heat transfer and improving equilibration times
  • High speed of heating and cooling so that more samples can be run in a fixed amount of time, increasing productivity
  • Heated cover assuring that tubes fit tightly in the wells
  • Oil-free operation
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