Bio-Rad Laboratories, Inc. - S3e™ Cell Sorter
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The S3e Cell Sorter is the first truly walk-away automated cell sorter. Real-time monitoring and smart features make cell sorting easier.
This compact system is equipped with either one or two lasers and up to four fluorescence detectors plus forward- and side-scatter detectors.
Among the many innovations of the S3e Cell Sorter, two key state-of-the-art advancements are ProDrop™ Technology and the AutoGimbal System:
ProDrop Technology produces extremely accurate and precise results by:
The highly sophisticated AutoGimbal System automates alignment of the nozzle tip and stream to optics by:
The affordable S3e Cell Sorter provides exceptional sort purity, recovery, and yield without compromising performance and sensitivity.
The system is ideal for sorting cells expressing fluorescent proteins such as eGFP, DsRed, and mCherry, or for cells labeled with fluorescent markers such as antibodies labeled with PE, PE tandems, and other fluorophores excited by the 488, 561, and 640 nm lasers.
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Features of S3e™ Cell Sorter
- Hands-free nozzle tip alignment — features the fully automatic AutoGimbal™ System, driven completely by software * Simplified instrument setup — fully automated drop delay calculation, stream alignment, and droplet break-off monitoring enable precise 1–4 color sorting with minimal training * Compact design — dimensions of only 70 × 65 × 65 cm (2.3 × 2.1 × 2.1 ft) including onboard fluidics and temperature control system * 2-way cell sorting — sort two different defined populations at the same time * High speed and high purity — sort cells fast while maintaining high sensitivity and purity * Intuitive interface — user-friendly ProSort™ Software enables effortless instrument control and sort logic definition
General Specifications
There are no General Specifications available.
Additional Specifications
Droplet frequency:
37–43 kHz
Sorting type:
True jet-in-air for high-performance sorting
Sorting rate:
No hardware limitations for sort rate, limited only by droplet frequency and application
Sorting purity:
>99% pure
Nozzle size:
100 µm
Sorting direction:
2-way sorting
Sorting collection:
Up to 5 x 5 ml sample tubes each direction
Up to 5 x 1.5 ml tubes each direction
Microscope slides
8-well strip each direction
Lasers:
Single laser: 488 nm 100 mW
Single-laser upgrade: 488 nm 100 mW plus 561 or 640 nm 100 mW
Dual laser: 488 and 561 or 640 nW 100 mW
Detection:
Forward scatter (FSC) with PMT
Side scatter (SSC) with PMT
Up to 4 fluorescence detectors with PMT
Minimum resolution: 0.5 µm
Sensitivity:
<125 MESF for FITC and PE
Temperature range:
Sample and collection temperature control system: 4–37°C
Peltier solid state system
Fluidics:
Onboard fluidics and dilution of 8x sheath fluid with deionized water
Data format:
FCS 3.1
Dimensions (W x D x H):
70 x 65 x 65 cm (2.3 x 2.1 x 2.1 ft)