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  4. Kapa Biosystems - KAPA Library Quantification Kits

Kapa Biosystems - KAPA Library Quantification Kits

Manufactured by  Kapa Biosystems
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for Next-Generation Sequencing

KAPA Library Quantification Kits contain all the reagents needed for the accurate, reliable and reproducible qPCR-based quantification of NGS libraries prior to pooling for capture or flow cell amplification. Kits contain KAPA SYBR® FAST qPCR Master Mix, optimized for high-performance SYBR Green I-based qPCR. The engineered KAPA SYBR FAST DNA Polymerase contained in the Master Mix amplifies GC- and AT-rich DNA fragments of different lengths with similar efficiency, making it the only qPCR Master Mix capable of accurate qPCR-based quantification of NGS libraries. The pre-diluted set of DNA Standards contained in each kit is carefully quality-controlled to ensure lot-to-lot consistency and avoid data drift over time. Optimized Primer Premixes ensure optimal PCR efficiency.
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Features of KAPA Library Quantification Kits
Accurately quantify PCR-competent sequencing templates 
  • qPCR only “counts” those library molecules that can be sequenced 
  • More consistent cluster densities or template-to-bead ratios enable optimal utilization of sequencing resources    Improve pooling for multiplexed sequencing 
  • Library concentrations determined by qPCR are more reliable compared to other methods 
  • The engineered KAPA SYBR FAST qPCR Master Mix enables accurate pooling of libraries with extreme GC-contents or longer inserts    Eliminate drift with carefully quality-controlled DNA Standards 
  • Prediluted DNA Standards eliminate quantification errors associated with preparation of standard curves using in-house standards 
  • KAPA DNA Standards undergo strict quality control to ensure lot-to-lot consistency and eliminate data drift over time    Improve throughput with automation 
  • Library quantification assay is compatible with 96- and 384-well format 
  • Library dilution, reaction setup and data analysis can be automated for HTP pipelines 
  • Learn More about KAPA Automated Solutions
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