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  4. Kapa Biosystems - KAPA Library Quantification Kits

Kapa Biosystems - KAPA Library Quantification Kits

Manufactured by  Kapa Biosystems
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for Next-Generation Sequencing

KAPA Library Quantification Kits contain all the reagents needed for the accurate, reliable and reproducible qPCR-based quantification of NGS libraries prior to pooling for capture or flow cell amplification. Kits contain KAPA SYBR® FAST qPCR Master Mix, optimized for high-performance SYBR Green I-based qPCR. The engineered KAPA SYBR FAST DNA Polymerase contained in the Master Mix amplifies GC- and AT-rich DNA fragments of different lengths with similar efficiency, making it the only qPCR Master Mix capable of accurate qPCR-based quantification of NGS libraries. The pre-diluted set of DNA Standards contained in each kit is carefully quality-controlled to ensure lot-to-lot consistency and avoid data drift over time. Optimized Primer Premixes ensure optimal PCR efficiency.
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Features of KAPA Library Quantification Kits

Accurately quantify PCR-competent sequencing templates 

  • qPCR only “counts” those library molecules that can be sequenced * More consistent cluster densities or template-to-bead ratios enable optimal utilization of sequencing resources 

    Improve pooling for multiplexed sequencing * Library concentrations determined by qPCR are more reliable compared to other methods * The engineered KAPA SYBR FAST qPCR Master Mix enables accurate pooling of libraries with extreme GC-contents or longer inserts 

    Eliminate drift with carefully quality-controlled DNA Standards * Prediluted DNA Standards eliminate quantification errors associated with preparation of standard curves using in-house standards * KAPA DNA Standards undergo strict quality control to ensure lot-to-lot consistency and eliminate data drift over time 

    Improve throughput with automation * Library quantification assay is compatible with 96- and 384-well format * Library dilution, reaction setup and data analysis can be automated for HTP pipelines * Learn More about KAPA Automated Solutions

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