Kapa Biosystems - KAPA Library Amplification Kits
Manufactured by Kapa Biosystems
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The gold standard for NGS library amplification.
KAPA Library Amplification Kits contain KAPA HiFi DNA Polymerase, a novel enzyme engineered for ultra-high fidelity and robustness using Kapa’s directed evolution technology platform.
KAPA HiFi has become the enzyme of choice for NGS library amplification due to its ability to amplify complex DNA populations with high fidelity, high efficiency, decreased PCR duplication rates and very low bias. This results in lower duplication rates and improved coverage of GC- and AT rich regions, promoters, low complexity and other challenging regions in all NGS library construction workflows requiring library amplification.
In addition to the standard library amplification formulation, KAPA HiFi is available in a unique real-time formulation, for applications that demand precise control over library amplification. A uracil-tolerant variant, KAPA HiFi Uracil+ is also available for the high-efficiency, high-fidelity, low-bias amplification of libraries constructed from bisulfite-treated DNA.
KAPA Library Amplification Kits for Illumina platforms now also include an optimally formulated Library Amplification Primer Mix.
KAPA HiFi has become the enzyme of choice for NGS library amplification due to its ability to amplify complex DNA populations with high fidelity, high efficiency, decreased PCR duplication rates and very low bias. This results in lower duplication rates and improved coverage of GC- and AT rich regions, promoters, low complexity and other challenging regions in all NGS library construction workflows requiring library amplification.
In addition to the standard library amplification formulation, KAPA HiFi is available in a unique real-time formulation, for applications that demand precise control over library amplification. A uracil-tolerant variant, KAPA HiFi Uracil+ is also available for the high-efficiency, high-fidelity, low-bias amplification of libraries constructed from bisulfite-treated DNA.
KAPA Library Amplification Kits for Illumina platforms now also include an optimally formulated Library Amplification Primer Mix.
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Features of KAPA Library Amplification Kits
Achieve the lowest amplification bias and duplication rates
Lower amplification bias result in improved representation of all library fragments and sequence regions
High-efficiency amplification leads to fewer amplification cycles and lower PCR duplicates
Less additional next-generation or Sanger sequencing needed to complete genomes
Improve coverage of GC- and AT-rich regions
Lower amplification bias improves coverage uniformity of GC- and AT-rich regions, promoters, low complexity and other challenging regions
Improved overall coverage and coverage uniformity observed on both Illumina and Ion Torrent sequencing platforms
Exercise precise control over library amplification
KAPA Real-Time Amplification Kits allow for library amplification to be observed in real time
Terminate amplification at the optimal point for individual samples
Optimize library amplification parameters for higher throughput workflows
Amplify NGS libraries with industry-leading fidelity
KAPA HiFi was engineered using directed evolution to have enhanced proofreading (3′-5′ exonuclease) activity
Industry-leading fidelity confirmed by 454 sequencing
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