BioTek - Lionheart™ FX Automated Live Cell Imager
Lionheart™ FX Automated Live Cell Imager with Augmented Microscopy™ is an all inclusive microscopy system,...
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Features of Lionheart™ FX Automated Live Cell Imager
Automated digital microscopy to 100x
Automated image capture, automated image analysis, automate movie making * Image-based and laser autofocus, auto LED intensity, auto exposure * Image whole organisms to subcellular details
Four imaging modes, multiple imaging processes * Brightfield, color brightfield, phase contrast and fluorescence * Z-stacking and z-projection, montage and stitching * Digital phase contrast
Gen5 3.0 Software tools * Integrated image capture, analysis, annotation and movie making on a single platform * Quick implementation, no extensive training required * Gen5 Image+ and Gen5 Image Prime for expanded image analysis
Kinetic live cell assay support * Environmental control cover contains temperature and gas * Humidity chamber supports long term live cell kinetics * Dual reagent injectors provide quick inject/image to observe fast reactions
Fully integrated and compact * Thoughtful design offers quick installation and setup * Tool-free connections for gas controller, injectors and environmental control cover * Filter cubes and objectives easily accessible from the front panel
General Specifications
There are no General Specifications available.
Additional Specifications
General
Microplate types
6- to 1536-well plates
Other labware supported
Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides
Support for labware up to 1.5" tall
Temperature control
Incubation to 40 °C with optional environmental control cover
Software
Gen5 3.0 Data Analysis Software included
Gen5 Image and Image+ Software available for advanced image analysis (option)
CO2 and O2 control (option)
0 - 20% CO2 control and 1 - 19% O2 control, with optional Gas Controller
Environment control cover
Top cover for light tight imaging and incubation control (option)
Stage control
High resolution lead screw driven stage with open access
Humidity control
Humidity chamber with rapid gas recharge (option)
Imaging System
Imaging mode
Fluorescence, brightfield, color brightfield, and phase contrast
Imaging method
Single color, multi-color, montage, time lapse, Z-stacking, burst mode
Light source
High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm)
Camera
16-bit gray scale, Sony CCD, 1.25 megapixel
Camera binning
Optional 2x2 binning for focus and/or image capture
Camera exposure range
5 milliseconds to 4 seconds
Image outputs available
Raw Images: 16-bit TIFF
Saved Images: TIF, JPG, BMP, PNG, EMF, GIF
Movies: MP4, WMV
Objective capacity
6 onboard, user-replaceable objectives
Objectives available
Fluorescence:
Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30;
20x, NA: .45; 40x, NA.60; 60x, NA .70
Oil: 60x, NA:1.42; 100x, NA: 1.40
Phase objectives available: 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA .60
Image filter cube capacity
Up to 4 onboard, user-replaceable cubes
Imaging filter cubes available
DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em)
Automated functions
Autofocus, user-trained autofocus, auto exposure, auto-LED intensity
Autofocus method
Image-based autofocus
Laser autofocus option
Image collection rate
Single Well Fastest Frame Rate Capture:
*Full Resolution: up to 10 frames per second for single color images
*2x2 Binning: up to 20 frames per second for single color images
Microscope stage control
Gen5 Software Control
Optional joystick controller
Reagent Injectors (Module Option)
Number
2 syringe pumps
Supported labware
6- to 384-well microplates, Petri and cell culture dishes, chamber slides
Dead volume
<1.65 mL with back flush
Dispense tip options
Aligned tip - aligned with optical path for dispensing for fast kinetic assays
Offset tip - dispensing is offset from the optical path
Dispense volume
5 - 1000 µL in 1 µL increment
Dispense accuracy
± 1 µL or 2%
Dispense precision
≤ 2% at 50 - 200 µL
Physical Characteristics
Power
250 Watts max.
Dimensions
With cover closed or without cover: 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm)
With cover fully open: 18.3" D, 17.9" W, 27.5" H (46.5 cm x 45.5 cm x 69.8 cm)
Weight
Without environmental control cover: 51 lbs (23.1 kg)
With environmental control cover: 58 lbs (26.3 kg)
Regulatory
CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available.