Teledyne Isco - CombiFlash® Rf+ Lumen™

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The smallest Flash system with integrated UV, UV-Vis and ELS detection

The CombiFlash Rf+ Lumen system enhances detection capabilities with an integrated evaporative light scattering detector (ELSD). In addition to UV or UV-Vis detection for absorbing compounds, the ELSD detects compounds with little or no chromophores such as carbohydrates, steroids, and lipids. The integrated ELSD conserves valuable bench space.

Large fraction capacity allows longer runs without changing racks. You don't need to divert time from other activities to watch your purification.
An automated injection valve lets you load samples and walk away - and it's self-cleaning to wash away materials that can contaminate the next run.

Whether you separate synthetic compounds, natural products, peptides, polymers, or other chemical separations; the CombiFlash Rf+ Lumen improves your compound's detectability.

The ELSD withdraws a miniscule portion of the liquid eluting from the chromatographic column into the detector module. This fluid is then nebulized using a stream of inert gas. This mixture forms an aerosol cloud of solvent droplets containing your compound. The aerosol cloud is then evaporated, leaving micro-particles of dried compound.

The gas stream carries these micro-particles to the detector where a laser light illuminates the gas stream. When compound is present, the particles cause the laser light to scatter, indicating its presence and creating a detector signal. This signal triggers the fraction collector to collect your compound.

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Features of CombiFlash® Rf+ Lumen™

Space saving design is smaller than other flash chromatography systems on the market, freeing scarce hood space for other uses.

Solvent and waste management prevents columns from drying and waste containers from hazardous overflows.

Large touch screen 10.4 inch (26.4 cm) puts the most used controls on a single, easy to read display.

RFID (Radio Frequency Identification) identifies columns and loads the appropriate method for the best separation. With reusable bonded-phase columns, the system tracks the number of runs on the column as well as the last solvent used, so you know whether you need to flush the column.

RFID identification of fraction collection racks sets the maximum fraction size to prevent overflows.

No waiting to access data - networking allows lets you connect to the CombiFlash® Rf 200 to monitor and remotely control your experiment, analyze data, and print to network printers.

Rapid column air purge prevents solvent from being discarded with the used column. It is better for the environment, safer in the lab, and eliminates messy column removal.

TLC Rf to Gradient calculator determines optimal separation parameters while minimizing run time.

Easy scale-up with built-in method-scaling means you won’t need to re-develop your method. You save time when you increase reaction size. You can even scale up to a 3 kg column run on a CombiFlash Torrent.

Back pressures to 200 psi for improved separations.

May be ordered in either variable UV 200 - 400 nm, or variable UV-Vis 200 - 800 nm configurations

General Specifications

Depth	17 in
Height	24 in
Width	14.1 in

Additional Specifications

Controller
10.4 inch touchscreen (26.4 cm), or external web browser via Ethernet. Data and current run can be accessed by up to 10 remote computers. Front panel USB port to save data and transfer files.
PeakTrak software for
On-screen method development; Real-time control - change method parameters during a run without pausing; Converting Rf values into solvent gradients; Method scaling -transfer method from one size column to another, from 4 g to 330 g; Converting methods to 3000 g columns on CombiFlash Torrent; Color-coded fraction identification.
Remote Wireless Access
Monitor system operation while away from the lab using iPad, iPhone, or iPod. Change conditions on the fly.
Programmable gradients
Linear and/or step gradients. Change profiles anytime during run, using "Click and drag" gradients. Pause gradient for isocratic hold.
Pumping system
Positive displacement pumps. 1-200 mL/min. 0-200 psi (when used with RediSep Rf columns)
Number of Solvents
Binary gradient with any two of four solvents; A third solvent can be used to add modifier at a fixed percentage.
Gradient Formation
Low pressure gradient formation, Accuracy <2% full scale; Linear, step, isocratic mixing capability
Auto-Method (RFID)
RFID column detection, RFID Test Tube Rack identification
Column sizes
4 g to 330 g.
Fraction collection
Internal fraction collector capacity- 13x100 mm tubes - 216, 16x125/150 mm tubes - 150, 18x150/180 mm tubes - 140, 25x150 mm tubes - 60, 40 mL scintillation vials - 60, 20 mL scintillation vials - 54, 480 mL bottles - 12.
Fraction Size
Determined by peak cutting and by user-defined maximum tube volume
Sample injection
Auto-inject with 6-position automated motorized sample loading valve; self-cleans at end of run; solid or liquid injection
Detection
Software selectable variable wavelength UV, 200-400 nm; optional variable wavelength UV-Vis, 200-800 nm; slope and threshold peak detection; detector range- 0.2-2.4 AUFS; determination of peak purity by wavelength ratios.
Detection
Evaporative Light Scattering (ELSD) +UV (UV-Vis optional)
Spray Chamber Temperature
Ambient to 120 °C
Drift Tube Temperature
10 to 70 °C
Gas Requirements
65 psi (4.6 bar) ± 5 psi (.3 bar) Nitrogen or other inert gas
Safety
Static dissipating tubing, vapor sensor, and column air purge.
Sample security
Auto-run extension at end of run, and over pressure sensor.
Solvent management
Four solvent and one waste fluid level monitoring
Certifications
CE

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