Organoid cultures exhibit cellular behaviors and morphologies similar to those found in vivo, but the adaptation of such models to study biochemical processes has been impeded by the challenge of separating intact organoids from extracellular proteins in the hydrogel. Commonly, proteases have been employed to degrade these extracellular proteins, but proteases also degrade proteins on the cell surface and protease activity may carry over into subsequent cultures or lysate preparations. 
 
Cultrex® Organoid Harvesting Solution provides a non-enzymatic method for depolymerizing extracellular matrix proteins to allow harvesting of intact organoids for passaging, cryopreservation, or biochemical analysis; and to facilitate PDX xenograft models.