MTI-GlobalStem - PluriQ™ G9™ Gene Editing System
Manufactured by MTI-GlobalStem
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The PluriQ™ G9™ Gene Editing System is a complete system for culturing and transfecting human pluripotent...
The PluriQ™ G9™ Gene Editing System is a complete system for culturing and transfecting human pluripotent stem cells for gene editing. The system includes the G9™ Maintenance Medium and G9™ VTN Recombinant (vitronectin) plate coating for culturing human induced pluripotent (iPS) or embryonic stem (hES) cells in a manner that maximizes transfection by the included EditPro™ Stem Transfection Reagent to transfect genome editing constructs. EditPro™ Stem is optimized for delivery of CRISPR/Cas9 editing via DNA Cas9 vectors, Cas9 mRNA with DS DNA (G-block), Cas9 mRNA with tracr/guide RNA, or Cas9 protein with tracr/guideRNA. It is effective for delivery of TALEN and ZFN editing tools as well. With such high efficiency delivery it is not necessary to include an antibiotic selection in the editing design.
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Features of PluriQ™ G9™ Gene Editing System
- High Efficiency Transfection of human pluripotent stem cells delivering DNA, RNA and/or protein * No need for antibiotic selection * Low toxicity and does not affect pluripotency * Easy to use – no special equipment required * Cost Effective – effective at low amounts of reagent resulting in low cost/well * Xeno-free, feeder-free culture of human pluripotent cells * Easy, gentle non-enzymatic passaging compatible with high throughput culture
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Additional Specifications
Recommended Use
Culture and transfection for genome editing of human ESC or iPSC.
Product Unit
Kit
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See individual components. Tested functionally together.
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For Research Use Only - Not intended for human or animal diagnostic or therapeutic uses