MTI-GlobalStem - EditPro™ Stem
Manufactured by MTI-GlobalStem
EditPro™ Stem Transfection Reagent is optimized for genome editing in human pluripotent stem cells. It is...
EditPro™ Stem Transfection Reagent is optimized for genome editing in human pluripotent stem cells. It is possible to co-transfect different substrates providing the flexibility needed for genome editing experiments. For example EditPro™ Stem provides efficient transfection of large DNA plasmids such as CRISPR/Cas9 vectors, RNA such as Cas9 mRNA co-transfected with tracr/guide RNA, or protein and RNA such as Cas9 protein with tracr/guide RNA. It also results in high efficiency transfection with TALENs and ZFN gene editing tools.
EditPro™ Stem Transfection Reagent is easy to use and can work with various human pluripotent stem cell culture conditions (media and matrices). The efficiency is maximized, however, when used in conjunction with the PluriQ™ G9™ Maintenance Medium Kit with the cells plated on G9™ VTN Human Recombinant as matrix as provided in the PluriQ™ G9™ Gene Editing System. The reason for the boost using this culturing format is that the cells grow more as a monolayer and therefore are more accessible to transfection resulting in even and high efficiency transfection with low toxicity.
EditPro™ Stem Transfection Reagent is easy to use and can work with various human pluripotent stem cell culture conditions (media and matrices). The efficiency is maximized, however, when used in conjunction with the PluriQ™ G9™ Maintenance Medium Kit with the cells plated on G9™ VTN Human Recombinant as matrix as provided in the PluriQ™ G9™ Gene Editing System. The reason for the boost using this culturing format is that the cells grow more as a monolayer and therefore are more accessible to transfection resulting in even and high efficiency transfection with low toxicity.
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Features of EditPro™ Stem
- High Efficiency – delivering DNA, RNA and/or protein * Low toxicity and does not affect pluripotency * Easy to use – no special equipment required * Cost Effective – effective at low amounts of reagent resulting in low cost/well
General Specifications
There are no General Specifications available.
Additional Specifications
Recommended Use
Transfection for genome editing of human ESC or iPSC.
Cell Source
Formulated Specifically for stem cells
Classification
Defined, animal origin-free
Size
0.25 mL
Sample Type
DNA, RNA and/or protein
Storage
Store refrigerated at 2-8°C Do Not Freeze
Shipping
Room Temperature
Other
For Research Use Only - Not intended for human or animal diagnostic or therapeutic uses