Waters - RapiGest SF Surfactant
Manufactured by Waters
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An Enabling Tool for Protein Digestions
The patented RapiGest SF Surfactant is a reagent used to enhance enzymatic digestion of proteins, both in-gel and in-solution. RapiGest SF helps solubilize proteins, making them more susceptible to enzymatic cleavage without inhibiting enzyme activity.
Unlike other commonly used denaturants, such as SDS or urea, RapiGest SF does not modify peptides or suppress protease activity. It is compatible with enzymes such as Trypsin, Lys-C, Asp-N, Glu-C, PNGaseF, and other enzymes.
Compatible for LC and MS analysis
Improves efficiency and speed of digestion
Degraded at low pH and removed from the sample
Simplifies preparation protocols and improves throughput of analysis
Unlike other commonly used denaturants, such as SDS or urea, RapiGest SF does not modify peptides or suppress protease activity. It is compatible with enzymes such as Trypsin, Lys-C, Asp-N, Glu-C, PNGaseF, and other enzymes.
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Features of RapiGest SF Surfactant
How RapiGest Works
[i]Rapi[/i]Gest SF is a mild denaturant that solubilizes and unfolds the substrate protein, making it more amenable to cleavage. Mild protein denaturation opens the protein structure and exposes the proteolytic sites to enzymatic cleavage. More complex denaturation of globular proteins can be accomplished by heating in a [i]Rapi[/i]Gest SF solution at elevated temperatures.
Compatibility with MS
[i]Rapi[/i]Gest SF is an acid labile surfactant that undergoes hydrolysis in acidic conditions. This unique feature can be utilized to remove the surfactant from solutions when desired. Acidic conditions break down [i]Rapi[/i]Gest SF to non-interfering by-products, therefore peptide ionization is not suppressed.
The RapiGest SF Advantage
[i]Rapi[/i]Gest SF shows significant advantage over other denaturants since it is not disruptive to endoprotease activity. It allows use of lesser amounts of expensive endoproteases. It is effective in a range of digestions such as Asp-N, Glu-C, Lys-C, PNGaseF and chymotrypsin digestions with the added advantage of significantly decreasing proteolytic digestion time, enabling same day digestions and analysis.
Increases peptide recovery and coverage
Facilitates the digestion of proteolytically resistant proteins
Increases solubility of hydrophobic proteins/peptides
General Specifications
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