Waters - Glycan Standard & Kits
Manufactured by Waters
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Specially formulated and high-quality
Waters Glycan Standards and Kits are for released N-glycan labeling. We offer standards in a wide variety of labels, 2-AB, 2-AA and RapiFluor-MS. In addition, we offer complete kits for both 2-AB and RapiFluor-MS labeling in a variety of sample sizes for low throughput to high throughput.
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Features of Glycan Standard & Kits
Glycan Performance Test Standard
The Glycan Performance Test Standard line is intended to be used as a benchmarking standard for ACQUITY UPLC BEH Glycan, 1.7 µm Columns.
Two versions of labeled standards for 2-AB (2-aminobenzamide and [i]Rapi[/i]Fluor-MS
Human IgG based
QC verified to contain the components needed to benchmark and evaluate HILIC columns
Dextran Calibration Ladder
In order to take full advantage of the BEH glycan column, Waters created a line of labeled Dextran Calibration Ladder Standards to assist glycan profiling. Glycan profile obtained from UPLC-HILIC/FLR systems can be calibrated against the labeled dextran ladder and assigned with glucose unit (GU) values. Waters Labeled Dextran Calibration Ladders are different than the existing commercial offerings. The average molecular weight of the glucose homopolymer is higher (~4,500 Dalton), therefore, the workable GU value range is twice as much as the other dextran ladder standards; the observed GU goes from 2 to 30. This feature improves the large glycan retention time assignment.
Labeled standards for 2-AA (anthranilic acid), 2-AB (2-aminobenzamide), and [i]Rapi[/i]Fluor-MS
Larger molecular weight range than typical commercially available ladders
Waters Ammonium Formate Solution
Quality of reagents is critical to mass spectrometry analysis. P/n 186007081 is a concentrated version of the 50mM Ammonium Formate buffer that is recommended to be used with the Biopharm/Glycan system, using the UPLC H-class and the ACQUITY UPLC BEH Glycan Column.
This buffer contains 10 ml of 5050 mM ammonium formate in a water w/ 3.8% formic acid matrix
This allows the end user to directly pour the concentrate into a 1 L bottle of LCMS grade water for the correct concentration of 50 mM at a pH of 4.4
Raw materials are all QC’d to ensure high quality MS friendly reagents which minimize adducts
The HILIC Advantage:
Hydrophilic Interaction Chromatography (HILIC) can be used to successfully improve retention of very polar species. This technique is rapidly growing for this application area. One reason for this is that this mode of separation is well suited for glycans due to their highly polar nature. The GlycoWorks HILIC SPE provides an efficient and reproducible mechanism for effective glycan cleanup and provides quantitative and consistent recoveries for a diverse range of N-glycans. Along with the GlycoWorks SPE Reagent Kit with a customized elution buffer this provides a very reproducible cleanup capturing species up to tetrasialyated.
Sample clean-up devices with HILIC (amino-propyle) sorbent
[i]High-throughput:[/i] GlycoWorks HILIC µElution SPE Plate (96-well)
[i]Single Use:[/i] GlycoWorks HILIC 1 cc (20 pack) and GlycoWorks HILIC 1 cc Flangeless Cartridges for the positive pressure manifold (20 pack)
GlycoWorks 2-AB N-Glycan Kits
The GlycoWorks Reagent Kit has been updated to include material for protein deglycosylation and complete 2-AB labeling as a cost effective alternative.
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