Waters - MassPREP Phosphopeptide Enrichment Kit

Manufactured by  Waters
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Enriching Phosphopeptides from Complex Matrices

Protein phosphorylation is one of the most common and most biologically important post translational modifications. This kit supplies the following: 
 
  • Optimized protocol to selectively enrich phosphopeptides from complex digests 
  • µElution plate format for SPE valuable 
  • Valuable for concentration of sample 
  • Control standard included
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    Features of MassPREP Phosphopeptide Enrichment Kit
    Kit Contents and Details  1) A 96 well µElution plate packed with a unique metal oxide sorbent.  2) A displacing reagent, which further improves the selectivity of the phosphopeptides, called the MassPREP Enhancer.  3) A standard test sample that contains enolase tryptic peptides mixed with 4 synthetic phosphopeptides.    Reproducible Data  In contrast to popular immobilized metal affinity chromatography (IMAC) technology, this isolation method does not require pre-loading the sorbent with a metal chelator. Therefore, the phophopeptide enrichment protocol is simplified. The robust method is provided by the sorbent that has a high native affinity towards phosphopeptides and the MassPREP Enhancer which yields superior selectivity of the sorbent towards phosphopeptides.    Standards to Help Benchmark  There are a suite of standards ranging from simple to complex, that consist of highly purified sample sets of synthetic phosphopeptides used to optimize phosphopeptide detection in LC-MS, LC-UV, and MALDI-MS analyses.     Includes 3 phosphopeptide standards ranging from simple to complex 
  • Highly Purified 
  • Synthetic 
  • Single to doubly phosphorylated
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