[u]Oasis PRiME HLB[/u]    Oasis PRiME HLB is the first-of-its-kind solid-phase extraction (SPE) sorbent that sets the new performance standard for routine analyses. The unique, patent-pending Oasis PRiME HLB Sorbent provides cleaner samples in less time and with less effort. Scientists desire sample preparation methods that are SIMPLE to follow while providing CLEANER sample eluates in the FASTEST time possible. Based on Oasis HLB Technology, Oasis PRiME HLB was developed to meet these needs by providing simpler methods, cleaner samples and faster protocols.        [u]Oasis PRiME HLB Advantages:[/u]   

SIMPLE three step and two step protocols with no SPE expertise required   

FASTER, more even flows across 96 well plates and cartridges with less plugging   

EVEN CLEANER samples with the removal of more than 95% of common matrix interferences such as proteins, salts and phospholipids        Oasis PRiME HLB is a patent pending reversed-phase sorbent that is designed to simplify SPE. This sorbent takes advantage of the desirable water-wettable and retention characteristics of Oasis HLB while providing cleaner samples in fewer steps, due to simpler protocols and the elimination of sorbent condition and equilibration steps. This is the ideal choice for routine analysis of reversed-phase SPE of acidic, basic and neutral analytes.        [u]The Most Versatile and Selective Solid-Phase Extraction Tool[/u]    Oasis® Sample Extraction Products are used by separation scientists across the globe to deliver unmatched purity, consistency and quality in their sample analysis. Oasis polymeric products deliver consistent retention and capacity -- even if the sorbent dries out. A number of formats are available, including our patented µElution plate. Oasis products deliver:       

Optimal sensitivity for small molecule and therapeutic peptide analysis   

Reduced matrix effects   

Unsurpassed selectivity   

Consistent retention and capacity, yielding the highest SPE recovery   

Wide selection of sorbents   

Broad array of formats        [u]Chemistry[/u]    Oasis Sorbents are available in 6 different solid-phase extraction (SPE) chemistries, providing a range of options for method development:        Oasis HLB is an all purpose, strongly hydrophilic, water-wettable polymer with a unique hydrophilic-lipophilic balance. Oasis HLB maintains high retention and capacity even if it runs dry after conditioning. This reversed-phase sorbent is ideal for acidic, basic and neutral analytes.         Oasis MCX is a mixed-mode, reversed-phase/strong cation-exchange,water-wettable polymer. Oasis MCX is selective for bases and stable in organic solvents.         Oasis MAX is a mixed-mode, reversed-phase/strong anion-exchange,water-wettable polymer. Oasis MAX is selective for acids and stable in organic solvents.        Oasis WCX is a mixed-mode, reversed-phase/weak cation-exchange, water-wettable polymer. Oasis WCX is used to retain and release strong bases and is stable in organic solvents.        Oasis WAX is amixed-mode, reversed-phase/weak anion-exchange, water-wettable polymer. Oasis WAX is used to retain and release strong acids and is stable in organic solvents.        [u]Methodology[/u]    Solid-phase extraction (SPE) method development starts with knowledge of the sample matrix—the nature and relative concentration of the analytes of interest and the potentially interfering endogenous compounds. The right sorbent and solvents are selected for load, wash, and elute steps, resulting in the creation of rugged, reliable, and selective protocols. Waters application chemists have devised several approaches to streamline this process.         The Oasis HLB method is a generic protocol used to isolate a broad range of acidic, basic, and neutral compounds from a wide spectrum of matrices.        [u]Oasis 2x4 Method[/u]    The Oasis 2x4 method for selection of SPE sorbent and protocol for acids, bases, and neutrals produces the cleanest extracts by eliminating matrix interferences and sample variability. Two protocols and four sorbents provide the flexibility to extract acids, bases, and neutrals with high SPE recoveries while removing matrix components that may interfere with analysis. Follow the simple steps outlined in this flow chart to achieve high recoveries and the cleanest extracts:       

Characterize the analyte (neutral, acid or base, pKa)   

Select one of the four Oasis sorbents   

Apply the indicated protocol (1 or 2)   

Determine SPE recoveries by LC analysis        [u]Formats Available[/u]    Oasis products come in a full range of device formats to meet your SPE requirements and to overcome your SPE challenges.        ΜElution Plates   

Patented ΜElution plate design   

No evaporation and reconstitution necessary due to elution volumes as low as 25 µL   

Ideal for SPE cleanup and analyte enrichment of small sample volumes   

Up to a 15x increase in concentration   

Compatible with most liquid-handling robotic systems for automated, reliable high throughput SPE (HT-SPE)        96-Well Extraction Plates   

Innovative award-winning two-stage well design   

High throughput and high recovery   

Available in 5 mg, 10 mg, 30 mg, and 60 mg per well   

Compatible with most liquid-handling robotic systems for automated, reliable high throughput SPE (HT-SPE)        Syringe-Barrel Cartridges   

Ultra-clean syringe barrel and frits   

Available in cartridges ranging from 1 cc to 60 cc   

Flangeless syringe-barrel cartridges available in 1 cc, 3 cc, and 6 cc sizes   

Also available as Plus-style cartridges with Luer inlet hub and outlet tip with 225 mg sorbent        Glass Cartridges   

Ultra-clean glass syringe with Teflon® frit   

For trace level detection and analysis at part-per-trillion (PPT) levels   

Available in 5 cc (200 mg) configuration        On-Line Columns and Cartridges   

For rugged, reproducible, and ultra-fast on-line analysis   

Wide choice of configurations, particle sizes, and sorbent chemistries   

Available with all six patented Oasis sorbents—PRiME HLB, HLB, MCX, MAX, WCX, and WAX   

High recovery and reproducible results for a wide range of compounds   

Cartridge format for use with Spark Holland Prospekt-2™/Symbiosis™ systems also available