Features

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General Specifications

There are no General Specifications available.

Additional Specifications

Technology:     
Silica-membrane technology     
     
Processing:     
Vacuum processing (e.g., using NucleoVac 24 Vacuum Manifold), centrifugation for elution     
     
Format:     
Snap-off column     
     
Endotoxin level:     
< 50 EU/µg DNA (EU = Endotoxin Units)     
     
Sample material:     
Up to 50 mL E. coli culture (OD600=5)     
     
Lysate clarification:     
NucleoSpin® Plasmid Filter Columns (2 min centrifugation)     
     
Vector size:     
< 25 kbp     
     
Typical yield:     
400–700 µg (50 mL culture, OD600=4, high-copy plasmid)     
     
A260/A280:     
1.8–1.9     
     
Elution volume:     
200–500 µL     
     
Preparation time:     
35 min/6 preps     
     
Binding capacity:     
1.5 mg