The AutoDG Instrument partitions each sample in a 96-well PCR plate into ~20,000 uniform nanoliter-sized droplets in which nucleic acid molecules are randomly distributed. Every droplet serves as an individual PCR reaction in Bio-Rad’s Droplet Digital (ddPCR™) PCR System. After partitioning, the plate is sealed with the PX1™ Plate Sealer, and PCR is run to end point in a thermal cycler. The droplets in each sample can then be analyzed using a QX100 or QX200 Droplet Reader.

• Eliminates user-to-user variability — creates more consistent droplet counts across users and plates, enabling you to fully exploit the precision of your ddPCR System
• Minimizes hands-on time — takes less than 5 minutes to load the instrument, and droplet generation requires no supervision

Required Instruments

• The Automated Droplet Generator requires the use of the following Bio-Rad instruments:
• QX200™ Droplet Reader
• C1000 Touch™ Thermal Cycler with 96–Deep Well Reaction Module
• PX1™ PCR Plate Sealer

Required Consumables

• DG32™ Automated Droplet Generator Cartridges for the AutoDG System
• Automated Droplet Generation Oil for Probes
• Automated Droplet Generation Oil for EvaGreen
• Pipet Tips for the AutoDG System
• Pipet Tip Waste Bin for the AutoDG System
• Droplet Reader Oil