Bio-Rad Laboratories, Inc. - Automated Droplet Generator
AutoDG™ Instrument for automated droplet generation of up to 96 samples, for use with the QX200™/QX100™ Droplet Digital™ PCR Systems
The AutoDG Instrument partitions each sample in a 96-well PCR plate into ~20,000 uniform nanoliter-sized droplets in which nucleic acid molecules are randomly distributed. Every droplet serves as an individual PCR reaction in Bio-Rad’s Droplet Digital (ddPCR™) PCR System. After partitioning, the plate is sealed with the PX1™ Plate Sealer, and PCR is run to end point in a thermal cycler. The droplets in each sample can then be analyzed using a QX100 or QX200 Droplet Reader.
- Eliminates user-to-user variability — creates more consistent droplet counts across users and plates, enabling you to fully exploit the precision of your ddPCR System
- Minimizes hands-on time — takes less than 5 minutes to load the instrument, and droplet generation requires no supervision
Required Instruments
- The Automated Droplet Generator requires the use of the following Bio-Rad instruments:
- QX200™ Droplet Reader
- C1000 Touch™ Thermal Cycler with 96–Deep Well Reaction Module
- PX1™ PCR Plate Sealer
Required Consumables
- DG32™ Automated Droplet Generator Cartridges for the AutoDG System
- Automated Droplet Generation Oil for Probes
- Automated Droplet Generation Oil for EvaGreen
- Pipet Tips for the AutoDG System
- Pipet Tip Waste Bin for the AutoDG System
- Droplet Reader Oil
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Features of Automated Droplet Generator
Features and Benefits of the Automated Droplet Generator Bio-Rad’s Automated Droplet Generator simplifies ddPCR workflow and makes digital PCR scalable and flexible:
- Guides setup and loading with large, color touch-screen interface and LED lighting
- Generates droplets for 96 ddPCR reactions in under 45 minutes
- One AutoDG Instrument can generate sufficient droplets to keep 4–5 droplet readers running continuously
- Provides operational flexibility:
- User-definable plate layout with increments of 8 wells
- HEPA-filtered enclosure reduces the possibility of contamination and allows the system to be placed in any lab environment
- Compatible with both QX100 and QX200 ddPCR workflows
General Specifications
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