Lumex Instruments - CE System CAPEL 105M
CAPEL-105M is a capillary electrophoresis system for fast and effective solutions for food industry, environmental...
PRINCIPLE OF OPERATION
High performance capillary electrophoresis is based on the differential migration of components of aqueous samples within a narrow fused silica capillary driven by an electric field. Separated solutes are quantitatively detected at the capillary outlet by a high sensitive optical system based on direct or indirect UV absorbance.
ANALYTICAL CHARACTERISTICS
Detectors
CAPEL-105M is equipped with a spectrophotometric detector with a wavelength range of 190 - 400 nm.
Light source
Deuterium lamp
Vials
Standard Eppendorf-type 1.5 ml, minimal sample volume 100 µl.
Injection modes
Sample injections by pressure, vacuum or electrokinetically.
Controlled pressure profile of injection with pressure values from – 99 mbar to + 99 mbar.
Electrokinetic injection at (+/-) 1 – 25 kV considering time for voltage ramping.
Programmable injection time / pressure / voltage.
Analysis
With a Voltage from -25 kV to +25 kV with a 1 kV step and an electronic polarity switch.
Current from 0 to 200 mA.
Elforun® software features
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Features of CE System CAPEL 105M
Extended instrumental options
Spectra scanning facilitates peak identification * Broad range of controlled injection pressure allows analysis of viscous samples * Reverse sample injection under vacuum: ultra-short analysis time (less than 1 min) and sample stacking to decrease detection limit
Precise and highly effective capillary thermostating provided by circulating liquid coolant (water)(± 0.1 C) * Extended range of applied buffers and increased efficiency in separation
Powerful software package "Elforun®" * Increased flexibility in performing analyses of various complexity * Any kind of complex runs are possible including those with pre-programming of changes in analysis conditions * Customized report, data export to other programs
Unique design of the capillary cassettes * Easy capillary change in just a few minutes * Lower Detection Limit due to the optimized optical scheme
General Specifications
Depth | 360 cm |
Height | 570 cm |
Width | 420 cm |
Power Requirements | 110/220 Vac, 50/60 Hz |
Weight | 25 kg |
Electropheresis Type | Capillary |
Additional Specifications
Detector:
Spectrophotometer
190-400 nm
Analysis:
Constant voltage (+/-) 1 - 25 kV in 1 kV steps
Automatic (electronic) polarity switch
Current 0 - 200 mkA
Pressure (+/-) 1-99 mbar
Programmable changing of wavelength, pressure and voltage during analysis
Injection:
By voltage
By pressure
By vacuum
Sampler:
Autosamplers for 10 inlet and 10 outlet vials
Rinsing:
With pressure ~1000 or 2000 mbar
Capillary:
Length: 30-100 cm
Internal diameter: 50, 75, 100 Μm
Capillary thermostating:
Liquid thermostating conducted at temperatures from 10 ºC below to 30ºC above ambient temperature (± 0.1 C)
Controlled parameters:
Wavelength
Pressure
Temperature
Voltage
Power consumption:
200 W