Lumex Instruments - CE System CAPEL®-205

Manufactured by  Lumex Instruments
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Capel®-205 is a new generation capillary electrophoresis system with advanced options and design. Its main features...

Capel®-205 is a new generation capillary electrophoresis system with advanced options and design. Its main features are:     
     
  • Extended autosampler capacity up to 59 positions, allowing to provide overnight analyses     
  • Standard centrifuge-type vials of 1.5mL volume (Eppendorf-type) closed with their original caps are used for all solutions     
  • Specially designed unit automatically opens vials just prior to their use, thus preventing evaporation and any kinds of cross-contamination     
  • New HV block provides voltage up to +/- 30 kV thus improving separation efficiency and decreasing analysis time     
  • Optionally – integrated unit to control the state of the inner capillary surface provides significantly improved migration time reproducibility especially when analyzing samples with complex matrices     
         
    Thus Capel®-205 can be the very best choice not only for R&D and low-scale analyses but also for the customers dealing routinely with dozens of samples per day.     
         
  • PRINCIPLE OF OPERATION     
    High performance capillary electrophoresis is based on the differential migration of components of aqueous samples within a narrow fused silica capillary driven by an electric field. Separated solutes are quantitatively detected at the capillary outlet by a high sensitive optical system based on direct or indirect UV absorbance. 
     
    ANALYTICAL CHARACTERISTICS 
    Detectors 
    CAPEL®-205 is equipped with a spectrophotometric detector with a wavelength range of 190 - 400 nm. 
     
    Light source 
    Deuterium lamp 
     
    Vials 
    Standard centrifuge-type (e.g.Eppendorf-type)1.5 ml, minimal sample volume 100 µl. 
     
    Injection modes 
    Sample injections by pressure, vacuum or electrokinetically. 
    Controlled pressure profile of injection with pressure values from – 100 mbar to + 100 mbar. 
    Electrokinetic injection at (+/-) 1 – 30 kV considering time for voltage ramping. 
    Programmable injection time / pressure / voltage. 
     
    Analysis 
    With a Voltage from -30 kV to +30 kV with a 1 kV step and an electronic polarity switch.  
    Current from 0 to 300 µA. 
     
    Elforun® software features 
    Combination of instrument controlling, data acquisition and data processing; 
    Real time electropherogram visualization; 
    Electropherogram data processing with overlay mode and basic mathematical operations; 
    Computation of electrophoresis system parameters; 
    Customized report output (hard copy and file), data exchanges with worksheets, databases and word processors; 
    Extensive data processing algorithm customization; 
    GLP and GALP compliance. 
     
    EQUIPMENT AND OPTIONS 
    Capillary electrophoresis system CAPEL®-205; 
    Spare capillary cassettes; 
    Elforun® software package; 
    Kits for analyses (by request); most of CE-kits of any manufacturers are compatible with Capel®-205.
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    Features of CE System CAPEL®-205

    Extended instrumental options      

    • Extended autosampler capacity up to 59 positions – hundreds of samples per months can be analyzed     * Integrated vials caps removal – all solutions are kept in the autosampler tightly closed excluding evaporation and cross-contamination     * New HV block provides voltage up to +/- 30 kV – separation efficiency is improved and analysis time is decreased     * Redesigned capillary cassette – length of the thermostated part of the capillary is increased to improve migration time reproducibility     * Spectra scanning facilitates peak identification     * Broad range of controlled injection pressure allows analysis of viscous samples     * Reverse sample injection under vacuum: ultra-short analysis time (less than 1 min) and sample stacking to decrease detection limit     

      Precise and highly effective capillary thermostating provided by circulating liquid coolant (water)(± 0.1 C)       Extended range of applied buffers and increased efficiency in separation     

      Powerful software package "Elforun®"      * Modified Elforun-205 to become even more user-friendly when operating with extended autosampler capacity     * Increased flexibility in performing analyses of various complexity     * Any kinds of complex runs are possible including those with pre-programming of changes in analysis conditions     * Customized report, data export to other programs     

      Unique design of the capillary cassettes      * Enlarged thermostatic part of the capillary improves migration time reproducibility     * Easy capillary change in just a few minutes     * Lower Detection Limit due to the optimized optical scheme

    General Specifications
    Depth 470 cm
    Height530 cm
    Width 410 cm
    Power Requirements 110/220 Vac, 50/60 Hz
    Weight30 kg
    Electropheresis TypeCapillary
    Additional Specifications

    Detector:     
    Spectrophotometer      
    190-400 nm     
         
    Analysis:     
    Constant voltage (+/-) 1 - 30 kV in 1 kV steps     
    Automatic (electronic) polarity switch     
    Current 0 - 300 mkA     
    Pressure (+/-) 1-100 mbar     
    Programmable changing of wavelength, pressure and voltage during analysis     
         
    Injection:     
    By voltage     
    By pressure     
    By vacuum     
         
    Sampler:     
    Autosamplers for 59 vials     
     
    Rinsing: 
    With pressure ~1000 or 2000 mbar     
     
    Capillary: 
    Length: 30-100 cm     
    Internal diameter: 50, 75, 100 Μm     
     
    Capillary thermostating: 
    Liquid thermostating conducted at temperatures from 10 ºC below to 30ºC above ambient temperature (± 0.1 C)  
        
    Controlled parameters: 
    Wavelength     
    Pressure     
    Temperature     
    Voltage

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