Easy-to-use kits require fewer manual steps, reducing hands-on time and risk for error. 
 
Gyrolab Protein A Kit is designed to detect native and recombinant protein A residuals in samples, such as antibody preparations after Protein A purification processes. In Gyros’ automated method, samples are acidified in the Gyrolab Mixing CD to dissociate protein A-IgG complexes. The amount of Protein A is then determined by a sandwich immunoassay. 
 

Quantifies native protein A, recombinant protein A variants and GE Healthcare’s MabSelect SuRe™ ligand 

Automates acid dissociation 

Provides results within 80 minutes 

Delivers precise, accurate data 
 
Standard assay protocol: 

Dilute samples to appropriate IgG concentration 

Dilute standards in series to set up seven points of diluted standard between 0.05 ng/ml and 40 ng/ml 

Dilute the capture antibody to 100 ug/ml and the detection antibody to 25 nM 

Prepare a microtiter plate with standards, capture and detection antibodies, acid dissociation buffers and wash buffers 

Load samples, reagents and Gyrolab Mixing CD(s) in a Gyrolab instrument 

First results available within 80 minutes