Horizon - EGFR Multiplex cfDNA
The EGFR Multiplex cfDNA Reference Standard Set is a cell line-derived, clinically relevant control that can be used to...
With this product, you are able to:
• Analyze the sensitivity and specificity of your assay
• Gain certainty of the limit of detection and limit of quantification
• Optimize and validate new EGFR-specific assays and routinely monitor their performance
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Features of EGFR Multiplex cfDNA
High-throughput platforms such as PCR-based assays and Next Generation Sequencing (NGS) give laboratories much greater power when profiling cfDNA samples. However, small quantities of DNA in combination with complex variants at low allelic frequencies represents one of the major challenges users encounter when profiling cfDNA.
Horizon Discovery has developed a comprehensive cfDNA Reference Standard range to support the development and continued validation of cfDNA assays. Our cfDNA products are derived from human cell lines and fragmented to an average size of 160 bp to closely resemble cfDNA extracted from human plasma.
The EGFR Multiplex cfDNA Reference Standard is supplied at 5%, 1%, 0.1% and 0% (EGFR Multiplex wild type) allelic frequencies and covers ten EGFR variants implicated in the responsiveness to EGFR tyrosine kinase inhibitors (EGFR-TKIs) and anti-EGFR monoclonal antibodies. This product covers clinically relevant SNPs, insertions and deletions in EGFR and can be used to optimize, validate or routinely monitor assay performance.
General Specifications
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Additional Specifications
Technical Data
Genes Covered EGFR
Allelic Frequencies 5%, 1%, 0.1% and 0% (EGFR Multiplex wild type)
Buffer Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0
Product Information
Fragment Size 160 bp
Unit Size 350 ng per vial
Concentration 20 ng/µl
Presence confirmed in parental cell line EGFR Q787Q, EGFR L844L
General Information
Storage 4°C
Expiry 12 months from the date of manufacture
Quality Control
Fragmentation Size D1000 DNA Screen Tape assay
Allelic Frequency Droplet Digital™ PCR
Quantification Qubit dsDNA BR Assay (post-fragmentation)