BioTek - Synergy H4
Manufactured by BioTek
Synergy H4 Hybrid Multi-Mode Microplate Reader
Synergy™ H4 with Hybrid Technology™ is a patent-pending multi-mode detector that combines the optical systems of Synergy Mx and Synergy 2 in one compact instrument. It is the ideal instrument for research and drug discovery applications when having to choose between flexibility and performance is not an option. The monochromator-based optics provides a high level of flexibility; any wavelength can be used from the low UV to the near infrared. The quadruple grating optical system, equipped with a variable bandpass selection system, is ideal for spectral scanning applications. The filter-based optics use dichroic mirrors for enhanced performance. This system is faster and more sensitive than the monochromator optics. A dual reagent dispenser option is available for inject and read assays such as flash luminescent assays and fluorescent ion channel assays. Additional read modes include: fluorescence polarization, time resolved fluorescence and AlphaScreen/AlphaLISA.
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Documents & Manuals
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Features of Synergy H4
- Hybrid Technology: high-performance filter-based detection, flexible monochromator-based detection.
- Detection Modes: Fluorescence intensity (FI), Fluorescence Polarization (FP), Time- Resolved Fluorescence (TRF), TR-FRET, High-Performance Luminescence, UV-Visible Absorbance, AlphaScreen / AlphaLISA.
- Compatible with Take3™ Multi-Volume Plate with 2 µL microspots for low volume assays
- Quadruple monochromator system and variable bandpass selection: Synergy H4 optics incorporates two double-grating monochromators. This quadruple design provides the best in spectral scanning performance and flexibility. Variable bandpass selection system adds flexibility.
- Deep blocking filters and dichroic mirrors: Synergy H4’s filter/dichroic combination provides the best possible performance for fluorescence, time resolved fluorescence, fluorescence polarization and AlphaScreen / AlphaLISA applications.
General Specifications
There are no General Specifications available.
Additional Specifications
Injection Volume Range: 5 - 1000 µL
Series Modals
BioTek - Absorbance
[+] View Model SpecificationsBandwidth | 2 nm (230-285 nm), 4 nm (>285 nm) |
Depth | 20.9 in |
Height | 15 in |
Detection Mode | Multi-label |
Width | 17 in |
Detection Method | Fluorescence, Time-Resolved Fluorescence, Fluorescence Polarization, AlphaScreen/AlphaLISA®, Luminescence, UV-Visible Absorbance |
Power Requirements | 250 Watts max. |
Sample Formats | Monochromator system 1- to 384-well plates Filter system 1- to 1536-well plates PCR plates Compatible with Take3™ Multi-Volume Plate with 2 µL microspots |
Lighting | Xenon Flash Lamp |
Weight | 78 lbs |
Read Method | End-point, kinetic, spectral scanning, well-area scanning |
Wavelength Range | 230 to 999 nm |
Scanning Speed | 96: 11 seconds, 384: 22 seconds |
Wavelength Selection | Monochromator |
Additional Model Specifications | Shaking: Yes Software: Gen5™ Included Control through USB or serial port Automation: Yes Absorbance Dynamic range: 0 - 4.0 OD Resolution: 0.0001 OD Pathlength correction: Yes Monochromator wavelength accuracy: +/- 2 nm Monochromator wavelength repeatability: +/- 0.2 nm OD accuracy: < 1% at 2.0 OD typical < 3% at 3.0 OD typical OD linearity: < 1% from 0 to 3.0 OD typical OD repeatability: < 0.5% at 2.0 OD typical Stray light: 0.03% at 230 nm typical Reagent Dispensers Number: 2 syringe pumps Dispense volume: 5 - 1000 µL in 1 µL increment Dead volume: 1.1 ml, 100 µL with back flush |
BioTek - Fluorescence Intensity
[+] View Model SpecificationsBandwidth | Monochromators: variable 9, 13.5, 17, 20 nm Filters: filter-dependant, from 5 nm to >100 nm |
Depth | 20.9 in |
Height | 15 in |
Detection Mode | Multi-label |
Width | 17 in |
Detection Method | Fluorescence, Time-Resolved Fluorescence, Fluorescence Polarization, AlphaScreen/AlphaLISA®, Luminescence, UV-Visible Absorbance |
Power Requirements | 250 Watts max. |
Sample Formats | Monochromator system 1- to 384-well plates Filter system 1- to 1536-well plates PCR plates Compatible with Take3™ Multi-Volume Plate with 2 µL microspots |
Lighting | Tungsten Halogen Lamp, High Energy Xenon Flash Lamp |
Weight | 78 lbs |
Read Method | End-point, kinetic, spectral scanning, well-area scanning |
Wavelength Range | 250 to 900 nm |
Scanning Speed | 96: 11 seconds, 384: 22 seconds, 1536: 42 seconds |
Wavelength Selection | Double grating monochromators (Top/Bottom) and, Deep blocking filters / dichroic mirrors (Top) |
Additional Model Specifications | Shaking: Yes Software: Gen5™ Included Control through USB or serial port Automation: Yes Fluorescence Intensity Sensitivity: Monochromators: Fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate) – Top Fluorescein 5 pM typical (0.5 fmol/well 384-well plate) – Bottom Filters/mirrors: Fluorescein 1 pM typical (0.1 fmol/well 384-well plate) Dynamic range: Monochromators: 5 decades, filters/mirrors: > 6 decades Detection system: Red shifted PMT for monochromator system Low Noise PMT for filter-based system Reagent Dispensers Number: 2 syringe pumps Dispense volume: 5 - 1000 µL in 1 µL increment Dead volume: 1.1 ml, 100 µL with back flush |
BioTek - Fluorescence Polarization
[+] View Model SpecificationsDepth | 20.9 in |
Height | 15 in |
Detection Mode | Multi-label |
Width | 17 in |
Detection Method | Fluorescence, Time-Resolved Fluorescence, Fluorescence Polarization, AlphaScreen/AlphaLISA®, Luminescence, UV-Visible Absorbance |
Power Requirements | 250 Watts max. |
Sample Formats | Monochromator system 1- to 384-well plates Filter system 1- to 1536-well plates PCR plates Compatible with Take3™ Multi-Volume Plate with 2 µL microspots |
Lighting | Tungsten Halogen |
Weight | 78 lbs |
Read Method | End-point, kinetic, spectral scanning, well-area scanning |
Wavelength Range | 400 to 700 nm |
Wavelength Selection | Deep blocking filters / dichroic mirrors (Top) |
Additional Model Specifications | Shaking: Yes Software: Gen5™ Included Control through USB or serial port Automation: Yes Sensitivity: 3 mP at 1 nM fluorescein typical Reagent Dispensers Number: 2 syringe pumps Dispense volume: 5 - 1000 µL in 1 µL increment Dead volume: 1.1 ml, 100 µL with back flush |
BioTek - Luminescence
[+] View Model SpecificationsDepth | 20.9 in |
Height | 15 in |
Detection Mode | Multi-label |
Width | 17 in |
Detection Method | Fluorescence, Time-Resolved Fluorescence, Fluorescence Polarization, AlphaScreen/AlphaLISA®, Luminescence, UV-Visible Absorbance |
Power Requirements | 250 Watts max. |
Sample Formats | Monochromator system 1- to 384-well plates Filter system 1- to 1536-well plates PCR plates Compatible with Take3™ Multi-Volume Plate with 2 µL microspots |
Weight | 78 lbs |
Read Method | End-point, kinetic, spectral scanning, well-area scanning |
Wavelength Range | 300 to 700 nm |
Additional Model Specifications | Shaking: Yes Software: Gen5™ Included Control through USB or serial port Automation: Yes Luminescence Sensitivity: 10 amol ATP typical (flash) Dynamic range: > 6 decades Reagent Dispensers Number: 2 syringe pumps Dispense volume: 5 - 1000 µL in 1 µL increment Dead volume: 1.1 ml, 100 µL with back flush |
BioTek - Time-Resolved Fluorescence
[+] View Model SpecificationsDepth | 20.9 in |
Height | 15 in |
Detection Mode | Multi-label |
Width | 17 in |
Detection Method | Fluorescence, Time-Resolved Fluorescence, Fluorescence Polarization, AlphaScreen/AlphaLISA®, Luminescence, UV-Visible Absorbance |
Power Requirements | 250 Watts max. |
Sample Formats | Monochromator system 1- to 384-well plates Filter system 1- to 1536-well plates PCR plates Compatible with Take3™ Multi-Volume Plate with 2 µL microspots |
Lighting | High Energy Xenon Flash Lamp |
Read Method | End-point, kinetic, spectral scanning, well-area scanning |
Wavelength Selection | Deep blocking filters / dichroic mirrors (Top) Double grating monochromator (Top / Bottom) |
Additional Model Specifications | Shaking: Yes Software: Gen5™ Included Control through USB or serial port Automation: Yes Sensitivity: Europium 60 fM typical with filters (6 amol/well in 384-well plate) Reagent Dispensers Number: 2 syringe pumps Dispense volume: 5 - 1000 µL in 1 µL increment Dead volume: 1.1 ml, 100 µL with back flush |
Videos
Synergy H4 Introduced at LabAutomation 2010