This product is Discontinued

Molecular Devices - SpectraMax M3/M4/M5/M5e

Manufactured by  Molecular Devices
Follow this Equipment

Your applications, your modes, your choice

The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Software. Detection modes include:      
          
  1. UV-Visible Absorbance (Abs)      
  2. Fluorescence Intensity (FI)      
  3. Luminescence (Lum)      
  4. Time-Resolved Fluorescence (TRF)      
  5. Fluorescence Polarization (FP)      
Active Questions & AnswersAsk a Question

Recent Questions & Answers

avatar placemark

Updated byNRuth

Microplate Readers Service ProvidersView All (11)

Request Support
Documents & Manuals

There are no Documents or Manuals available.

Features of SpectraMax M3/M4/M5/M5e
  • Upgradeable platform for changing lab needs      
  • Three-mode cuvette port for assay development      
  • Dual monochromator tunability      
  • Automated Absorbance Pathlength Correction      
  • Endpoint, kinetic, spectral and well-scanning read types      
  • Comprehensive Data Analysis with SoftMax Pro Software      
  • Validation & compliance      
  • Robotics compatibility      

Dual Monochromators for assay flexibility      

With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. The optical systems use two scanning monochromators so the user can determine optimal excitation and emission settings, resulting in assay performance similar to that of dedicated single-mode readers.      

Patented pathlength correction for better absorbance      

Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no termperature dependency using the patented PathCheck® Sensor technology. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. This eliminates the need for standard curves, and, for compounds with known absorptive properties, enables users to calculate concentrations directly from absorbance.      

Unique optical characteristics      

  1. The reference diodes enable elimination of measurement noise due to slight fluctuations in excitation light intensity.      
  2. The angled emission beam improves signal-to-noise, especially in narrow Stokes shift fluorophores, by reducing stray light.      
  3. Elliptical mirrors are used instead of lenses for maximum transmission with minimal wavelength distortion.      
  4. Top-quality UV-grade fibers give the highest light transmission down to even the lowest wavelengths.      

Assay collaboration for ease of setup      

Molecular Devices has collaborated with various assay partners to optimize and validate homogeneous and heterogeneous biochemical- or cell-based assay performance on the SpectraMax platform. To support these assays, we provide application notes as well as ready-to-run protocols in our SoftMax Pro Software. Some of our featured partner assays include HTRF assays from Cisbio Bioassays and LanthaScreen® TR-FRET assays from Invitrogen.

General Specifications

There are no General Specifications available.

Additional Specifications

General specifications      
Power Consumption: < 420 watts      
Power Source: 100-240 VAC, 3 A, 50/60 Hz      
Robotic Compatible: Yes      
      
General Photometric Performance      
Sample formats: 6, 12, 24, 48, 96, 384 wells      
Light source: Xenon Flash Lamp (1 joule/flash)      
Detectors: 2 photomultiplier tubes      
Shake time: 0 to 999 seconds      
Temperature control: 2 °C above ambient to 60 °C      
Temperature uniformity: < 1°C at 37°C set point      
Temperature accuracy: ±1°C at 37°C set point      
Endpoint reading: All modes      
Kinetic reading: All modes      
Spectral scanning: All modes      
Well scanning: Abs, FI, TRF, Lum      
      
Typical Read Times      
Absorbance: 96 wells in 18 seconds, 384 wells in 49 seconds      
Fluorescence intensity: 96 wells in 17 seconds, 384 wells in 48 seconds      
Fluorescence polarization: 96 wells in 42 seconds, 384 wells in 2 minutes, 3 seconds       
Time-resolved fluorescence: 96 wells in 17 seconds, 384 wells in 48 seconds      
Luminescence: 96 wells in 2 minutes, 384 wells in 7 minutes      
      
Absorbance Photometric Performance      
Reading capabilities: Cuvette or microplate      
Wavelength range: 200-1000 nm      
Wavelength selection: Monochromator, tunable in 1.0 nm increments      
Wavelength bandwidth: < 4.0 nm      
Wavelength accuracy: ±2.0 nm      
Wavelength repeatability: ±0.2 nm      
Photometric range: 0-4.0 OD      
Photometric resolution: 0.001 OD      
Photometric accuracy (microplate): < ±0.006 OD ±1.0%, 0-2 OD      
Photometric accuracy (cuvette): < ±0.005 OD ±1.0%, 0-2 OD      
Photometric precision: < ±0.003 OD ±1.0%, 0-2 OD      
Stray light: < 0.05% @ 230 nm      
      
Fluorescence Intensity Performance      
Reading capabilities: Cuvette or top or bottom of a microplate      
Wavelength range: 250-850 nm      
Wavelength selection: Monochromators, tunable in 1.0 nm increments      
Bandwidth (Ex, Em): 9 nm, 15 nm      
Sensitivity: < 5 pM fluorescein in 96 wells or cuvette, < 20 pM in 384 wells      
      
Luminescence Performance      
Reading capabilities: Cuvette or top or bottom of a microplate      
Wavelength selection: All wavelengths or with selected wavelengths      
Wavelength range: 250-850 nm      
Sensitivity: < 2 fg/well lower detection limit for firefly luciferase in 96- and 384-well top read      
Cross-talk: < 0.5% in 96- and 384-well microplates      
      
Time-resolved Fluorescence Performance (M4, M5, M5e only)      
Reading capabilities: Top or bottom of a microplate      
Wavelength range: 250-850 nm      
Wavelength selection: Monochromators, tunable in 1.0 nm increments      
Bandwidth (Ex, Em): 9 nm, 15 nm      
Precision data collection: 1-100 flashes, delay of 0-600 µsec before read, integration time selectable between 50-1500 µsec      
Sensitivity: 100 fM europium in 96 or 384 wells with top-read      
SpectraMax M5e reader only: Certified to Cisbio Bioassays' HTRF assays performance Specifications      
      
Fluorescence Polarization Performance      
Wavelength range: 400-750 nm      
Wavelength selection: Monochromators, tunable in 1.0 nm increments      
Bandwidth (Ex, Em): 9 nm, 15 nm      
Precision: < 5 mP standard deviation at 1 nM fluorescein in 96 and 384 wells

Series Modals

Molecular Devices - Absorbance

[+] View Model Specifications

Molecular Devices - Fluorescence Intensity

[+] View Model Specifications

Molecular Devices - Fluorescence Polarization

[+] View Model Specifications

Molecular Devices - Luminescence

[+] View Model Specifications

Molecular Devices - Time-Resolved Fluorescence

[+] View Model Specifications
NewsView All Articles

Looking for New or Used Equipment?

Shop on LabX

Shop on Labx.com