Molecular Devices - SpectraMax M3/M4/M5/M5e
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Your applications, your modes, your choice
- UV-Visible Absorbance (Abs)
- Fluorescence Intensity (FI)
- Luminescence (Lum)
- Time-Resolved Fluorescence (TRF)
- Fluorescence Polarization (FP)
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Documents & Manuals
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Features of SpectraMax M3/M4/M5/M5e
- Upgradeable platform for changing lab needs
- Three-mode cuvette port for assay development
- Dual monochromator tunability
- Automated Absorbance Pathlength Correction
- Endpoint, kinetic, spectral and well-scanning read types
- Comprehensive Data Analysis with SoftMax Pro Software
- Validation & compliance
- Robotics compatibility
Dual Monochromators for assay flexibility
With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. The optical systems use two scanning monochromators so the user can determine optimal excitation and emission settings, resulting in assay performance similar to that of dedicated single-mode readers.
Patented pathlength correction for better absorbance
Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no termperature dependency using the patented PathCheck® Sensor technology. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. This eliminates the need for standard curves, and, for compounds with known absorptive properties, enables users to calculate concentrations directly from absorbance.
Unique optical characteristics
- The reference diodes enable elimination of measurement noise due to slight fluctuations in excitation light intensity.
- The angled emission beam improves signal-to-noise, especially in narrow Stokes shift fluorophores, by reducing stray light.
- Elliptical mirrors are used instead of lenses for maximum transmission with minimal wavelength distortion.
- Top-quality UV-grade fibers give the highest light transmission down to even the lowest wavelengths.
Assay collaboration for ease of setup
Molecular Devices has collaborated with various assay partners to optimize and validate homogeneous and heterogeneous biochemical- or cell-based assay performance on the SpectraMax platform. To support these assays, we provide application notes as well as ready-to-run protocols in our SoftMax Pro Software. Some of our featured partner assays include HTRF assays from Cisbio Bioassays and LanthaScreen® TR-FRET assays from Invitrogen.
General Specifications
There are no General Specifications available.
Additional Specifications
General specifications
Power Consumption: < 420 watts
Power Source: 100-240 VAC, 3 A, 50/60 Hz
Robotic Compatible: Yes
General Photometric Performance
Sample formats: 6, 12, 24, 48, 96, 384 wells
Light source: Xenon Flash Lamp (1 joule/flash)
Detectors: 2 photomultiplier tubes
Shake time: 0 to 999 seconds
Temperature control: 2 °C above ambient to 60 °C
Temperature uniformity: < 1°C at 37°C set point
Temperature accuracy: ±1°C at 37°C set point
Endpoint reading: All modes
Kinetic reading: All modes
Spectral scanning: All modes
Well scanning: Abs, FI, TRF, Lum
Typical Read Times
Absorbance: 96 wells in 18 seconds, 384 wells in 49 seconds
Fluorescence intensity: 96 wells in 17 seconds, 384 wells in 48 seconds
Fluorescence polarization: 96 wells in 42 seconds, 384 wells in 2 minutes, 3 seconds
Time-resolved fluorescence: 96 wells in 17 seconds, 384 wells in 48 seconds
Luminescence: 96 wells in 2 minutes, 384 wells in 7 minutes
Absorbance Photometric Performance
Reading capabilities: Cuvette or microplate
Wavelength range: 200-1000 nm
Wavelength selection: Monochromator, tunable in 1.0 nm increments
Wavelength bandwidth: < 4.0 nm
Wavelength accuracy: ±2.0 nm
Wavelength repeatability: ±0.2 nm
Photometric range: 0-4.0 OD
Photometric resolution: 0.001 OD
Photometric accuracy (microplate): < ±0.006 OD ±1.0%, 0-2 OD
Photometric accuracy (cuvette): < ±0.005 OD ±1.0%, 0-2 OD
Photometric precision: < ±0.003 OD ±1.0%, 0-2 OD
Stray light: < 0.05% @ 230 nm
Fluorescence Intensity Performance
Reading capabilities: Cuvette or top or bottom of a microplate
Wavelength range: 250-850 nm
Wavelength selection: Monochromators, tunable in 1.0 nm increments
Bandwidth (Ex, Em): 9 nm, 15 nm
Sensitivity: < 5 pM fluorescein in 96 wells or cuvette, < 20 pM in 384 wells
Luminescence Performance
Reading capabilities: Cuvette or top or bottom of a microplate
Wavelength selection: All wavelengths or with selected wavelengths
Wavelength range: 250-850 nm
Sensitivity: < 2 fg/well lower detection limit for firefly luciferase in 96- and 384-well top read
Cross-talk: < 0.5% in 96- and 384-well microplates
Time-resolved Fluorescence Performance (M4, M5, M5e only)
Reading capabilities: Top or bottom of a microplate
Wavelength range: 250-850 nm
Wavelength selection: Monochromators, tunable in 1.0 nm increments
Bandwidth (Ex, Em): 9 nm, 15 nm
Precision data collection: 1-100 flashes, delay of 0-600 µsec before read, integration time selectable between 50-1500 µsec
Sensitivity: 100 fM europium in 96 or 384 wells with top-read
SpectraMax M5e reader only: Certified to Cisbio Bioassays' HTRF assays performance Specifications
Fluorescence Polarization Performance
Wavelength range: 400-750 nm
Wavelength selection: Monochromators, tunable in 1.0 nm increments
Bandwidth (Ex, Em): 9 nm, 15 nm
Precision: < 5 mP standard deviation at 1 nM fluorescein in 96 and 384 wells
Series Modals
Molecular Devices - Absorbance
[+] View Model Specifications| Bandwidth | < 4.0 nm |
| Detection Mode | Multi-label |
| Detector | 2 photomultiplier tubes |
| Lighting | Xenon Flash Lamp (1 joule/flash) |
| Photometric Range | 0-4.0 OD |
| Read Method | Cuvette or microplate. Endpoint, kinetic, spectral scanning, well scanning |
| Sample Formats | 6, 12, 24, 48, 96, 384 wells |
| Scanning Speed | 96 wells in 18 seconds, 384 wells in 49 seconds |
| Wavelength Range | 200 to 1000 nm |
| Wavelength Selection | Monochromator, tunable in 1.0 nm increments |
| Depth | 15.3 in |
| Height | 8.6 in |
| Width | 22.8 in |
| Power Requirements | 100-240 VAC, 3 A, 50/60 Hz, <420 W consumption |
| Weight | 36 lbs |
| Additional Model Specifications | Robotic Compatible: Yes General Photometric Performance Shake time: 0 to 999 seconds Temperature uniformity: < 1°C at 37°C set point Temperature accuracy: ±1°C at 37°C set point Absorbance Photometric Performance Wavelength accuracy: ±2.0 nm Wavelength repeatability: ±0.2 nm Photometric resolution: 0.001 OD Photometric accuracy (microplate): < ±0.006 OD ±1.0%, 0-2 OD Photometric accuracy (cuvette): < ±0.005 OD ±1.0%, 0-2 OD Photometric precision: < ±0.003 OD ±1.0%, 0-2 OD Stray light: < 0.05% @ 230 nm |
Molecular Devices - Fluorescence Intensity
[+] View Model Specifications| Bandwidth | Ex: 9 nm, Em: 15 nm |
| Detection Mode | Multi-label |
| Detector | 2 photomultiplier tubes |
| Lighting | Xenon Flash Lamp (1 joule/flash) |
| Read Method | Cuvette or top or bottom of a microplate. Endpoint, kinetic, spectral scanning, well scanning |
| Sample Formats | 6, 12, 24, 48, 96, 384 wells |
| Scanning Speed | 96 wells in 17 seconds, 384 wells in 48 seconds |
| Wavelength Range | 250 to 850 nm |
| Wavelength Selection | Monochromators, tunable in 1.0 nm increments |
| Depth | 15.3 in |
| Height | 8.6 in |
| Width | 22.8 in |
| Power Requirements | 100-240 VAC, 3 A, 50/60 Hz, <420 W |
| Weight | 36 lbs |
| Additional Model Specifications | Robotic Compatible: Yes Sensitivity: < 5 pM fluorescein in 96 wells or cuvette, < 20 pM in 384 wells General Photometric Performance Shake time: 0 to 999 seconds Temperature uniformity: < 1°C at 37°C set point Temperature accuracy: ±1°C at 37°C set point |
Molecular Devices - Fluorescence Polarization
[+] View Model Specifications| Bandwidth | Ex: 9 nm, Em: 15 nm |
| Detection Mode | Multi-label |
| Detector | 2 photomultiplier tubes |
| Lighting | Xenon Flash Lamp (1 joule/flash) |
| Read Method | Endpoint, kinetic, spectral scanning |
| Sample Formats | 6, 12, 24, 48, 96, 384 wells |
| Scanning Speed | 96 wells in 42 seconds, 384 wells in 2 minutes, 3 seconds |
| Wavelength Range | 400 to 750 nm |
| Wavelength Selection | Monochromators, tunable in 1.0 nm increments |
| Depth | 15.3 in |
| Height | 8.6 in |
| Width | 22.8 in |
| Power Requirements | 100-240 VAC, 3 A, 50/60 Hz, <420 W consumption |
| Weight | 36 lbs |
| Additional Model Specifications | Robotic Compatible: Yes Precision: < 5 mP standard deviation at 1 nM fluorescein in 96 and 384 wells General Photometric Performance Shake time: 0 to 999 seconds Temperature uniformity: < 1°C at 37°C set point Temperature accuracy: ±1°C at 37°C set point |
Molecular Devices - Luminescence
[+] View Model Specifications| Detection Mode | Multi-label |
| Detector | 2 photomultiplier tubes |
| Lighting | Xenon Flash Lamp (1 joule/flash) |
| Read Method | Cuvette or top or bottom of a microplate. Endpoint, kinetic, spectral scanning, well scanning |
| Sample Formats | 6, 12, 24, 48, 96, 384 wells |
| Scanning Speed | 96 wells in 2 minutes, 384 wells in 7 minutes |
| Wavelength Range | 250 to 850 nm |
| Wavelength Selection | All wavelengths or with selected wavelengths |
| Depth | 15.3 in |
| Height | 8.6 in |
| Width | 22.8 in |
| Power Requirements | 100-240 VAC, 3 A, 50/60 Hz, <420 W consumption |
| Weight | 36 lbs |
| Additional Model Specifications | Robotic Compatible: Yes General Photometric Performance Shake time: 0 to 999 seconds Temperature uniformity: < 1°C at 37°C set point Temperature accuracy: ±1°C at 37°C set point Luminescence Performance Sensitivity: < 2 fg/well lower detection limit for firefly luciferase in 96- and 384-well top read Cross-talk: < 0.5% in 96- and 384-well microplates |
Molecular Devices - Time-Resolved Fluorescence
[+] View Model Specifications| Bandwidth | Ex: 9 nm, Em: 15 nm |
| Detection Mode | Multi-label |
| Detector | 2 photomultiplier tubes |
| Lighting | Xenon Flash Lamp (1 joule/flash) |
| Read Method | Top or bottom of a microplate. Endpoint, kinetic, spectral scanning, well scanning |
| Sample Formats | 6, 12, 24, 48, 96, 384 wells |
| Scanning Speed | 96 wells in 17 seconds, 384 wells in 48 seconds |
| Wavelength Range | 250 to 850 nm |
| Wavelength Selection | Monochromators, tunable in 1.0 nm increments |
| Depth | 15.3 in |
| Height | 8.6 in |
| Width | 22.8 in |
| Power Requirements | 100-240 VAC, 3 A, 50/60 Hz, <420 W consumption |
| Weight | 36 lbs |
| Additional Model Specifications | Robotic Compatible: Yes General Photometric Performance Shake time: 0 to 999 seconds Temperature uniformity: < 1°C at 37°C set point Temperature accuracy: ±1°C at 37°C set point Time-resolved Fluorescence Performance (M4, M5, M5e only) Precision data collection: 1-100 flashes, delay of 0-600 µsec before read, integration time selectable between 50-1500 µsec Sensitivity: 100 fM europium in 96 or 384 wells with top-read SpectraMax M5e reader only: Certified to Cisbio Bioassays' HTRF assays performance Specifications |