Bio-Rad Laboratories, Inc. - xMark

Manufactured by  Bio-Rad Laboratories, Inc.
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Extending the Limits of Microplate Reading With Remarkable Flexibility

With its unique imaging capabilities, monochromator design, and spectral scanning feature (which eliminates the need for filters), the xMark microplate absorbance spectrophotometer can find the best wavelength within a broad range for any photometric application. The xMark spectrophotometer is also flexible enough to read all standard microplates, from 6- to 1,536-well formats. Using Microplate Manager software, the xMark spectrophotometer can perform a wide range of end-point and kinetic applications with spectral scanning within low UV to infrared wavelengths. Extensive data analysis includes comprehensive curve-fit models and automated data export in single-plate or batch formats.
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Features of xMark
  • Unique monochromator design with wide spectral range of 200–1,000 nm and 1 nm step size   

  • Capability to scan standard 6- to 1,536-well microplates and custom plates  

  • Unique well and plate imaging   

  • Built-in incubator* with continuous temperature display using Microplate Manager® software  

  • Built-in shaker* with variable mixing speed and linear or orbital mixing mode  

  • Single and dual wavelength detection  

  • Motorized tray for plate loading and retrieval   

  • Fast plate reading — 9 sec at single wavelength detection for a 96-well plate  

  • Microplate Manager software controlled (PC or Mac) via USB2 port  

  • Reports with Checkmark™ reader performance validation kit  

  • Incubator and shaker can be used independently from other instrument functions.  

Benefits  

  • Detection, analysis, and quantitation of substances with absorbance spectra in the UV range (DNA) to the infrared range (water)  
  • High throughput with sample volumes below 5 µl  
  • No restrictions on sample type, volume, or throughput  
  • Wide range of applications, from determiningHLA antigens serologically to testing wine quality  
  • Intensity maps for analysis of absorbance variations in a single well or entire plate  
  • Enhanced detection sensitivity  
  • Thermal regulation of assay reactions  
  • Improved sensitivity and activity of assays  
  • Incubation of plates prior to detection   
  • Accurate absorbance readout for entire well volume   
  • Minimum variability of assays for different sample types and liquid- or solid-state reactions   
  • Assay preparation and repeated mixing   
  • Detection of reaction products with high specificity while eliminating background and noise  
  • Minimized environmental exposure  
  • Precision in kinetic assays and rapidly developing colorimetric reactions   
  • Protocol customization and data reduction options  
  • Comprehensive data analysis  
  • Accommodation of various validation protocols, including installation qualification and operational qualification (IQ/OQ)
General Specifications
BandwidthOptical: 5 nm
Depth 17.3 in
Shaking1
Height8.6 in
Detection ModeAbsorbance
Width 21.4 in
Weight61 lbs
Wavelength Range200 to 1000 nm
Scanning Speed8 sec/96-well/single wavelength; 15 sec/384-well/single wavelength; 35 sec/1,536-well/single wavelength
Microplate Size1536-well
SoftwareExternal
Temperature Control 1
Additional Specifications

Optical  
Wavelength Accuracy (minimum): ±2.0nm  
Wavelength Repeatability: ±2.0 nm  
Photometric methods: single and dual wavelengths  
Monochromator step size: 1 nm  
Imaging Resolution: 0.5,0.75,1.0 mm  
  
Performance  
Indication range: 0-4.0 OD  
Resolution: 0.001 OD  
Linearity: <2.0%, 0-3.0 OD at 405 nm  
Reproducibility: 96-well plates, <1.0% or 0.005, 0-3.0 OD at 405 nm  
Stability and drift: <0.010 at OD = 1 at 490 nm

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