Bio-Rad Laboratories, Inc. - xMark
Extending the Limits of Microplate Reading With Remarkable Flexibility
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Features of xMark
Unique monochromator design with wide spectral range of 200–1,000 nm and 1 nm step size
Capability to scan standard 6- to 1,536-well microplates and custom plates
Unique well and plate imaging
Built-in incubator* with continuous temperature display using Microplate Manager® software
Built-in shaker* with variable mixing speed and linear or orbital mixing mode
Single and dual wavelength detection
Motorized tray for plate loading and retrieval
Fast plate reading — 9 sec at single wavelength detection for a 96-well plate
Microplate Manager software controlled (PC or Mac) via USB2 port
Reports with Checkmark™ reader performance validation kit
Incubator and shaker can be used independently from other instrument functions.
Benefits
- Detection, analysis, and quantitation of substances with absorbance spectra in the UV range (DNA) to the infrared range (water)
- High throughput with sample volumes below 5 µl
- No restrictions on sample type, volume, or throughput
- Wide range of applications, from determiningHLA antigens serologically to testing wine quality
- Intensity maps for analysis of absorbance variations in a single well or entire plate
- Enhanced detection sensitivity
- Thermal regulation of assay reactions
- Improved sensitivity and activity of assays
- Incubation of plates prior to detection
- Accurate absorbance readout for entire well volume
- Minimum variability of assays for different sample types and liquid- or solid-state reactions
- Assay preparation and repeated mixing
- Detection of reaction products with high specificity while eliminating background and noise
- Minimized environmental exposure
- Precision in kinetic assays and rapidly developing colorimetric reactions
- Protocol customization and data reduction options
- Comprehensive data analysis
- Accommodation of various validation protocols, including installation qualification and operational qualification (IQ/OQ)
General Specifications
Bandwidth | Optical: 5 nm |
Depth | 17.3 in |
Shaking | 1 |
Height | 8.6 in |
Detection Mode | Absorbance |
Width | 21.4 in |
Weight | 61 lbs |
Wavelength Range | 200 to 1000 nm |
Scanning Speed | 8 sec/96-well/single wavelength; 15 sec/384-well/single wavelength; 35 sec/1,536-well/single wavelength |
Microplate Size | 1536-well |
Software | External |
Temperature Control | 1 |
Additional Specifications
Optical
Wavelength Accuracy (minimum): ±2.0nm
Wavelength Repeatability: ±2.0 nm
Photometric methods: single and dual wavelengths
Monochromator step size: 1 nm
Imaging Resolution: 0.5,0.75,1.0 mm
Performance
Indication range: 0-4.0 OD
Resolution: 0.001 OD
Linearity: <2.0%, 0-3.0 OD at 405 nm
Reproducibility: 96-well plates, <1.0% or 0.005, 0-3.0 OD at 405 nm
Stability and drift: <0.010 at OD = 1 at 490 nm