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BMG LABTECH - Omega Series

Manufactured by  BMG LABTECH
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UV/Vis Absorbance Spectrophotometer Microplate Reader; complete spectrum within 1 second

The Omega series is BMG LABTECHs 5th generation of multidetection life science microplate readers. They employ Tandem Technology (TT), which uses an ultra-fast UV/Vis spectrometer for absorbance and high performance filters for fluorescence. The Omega offers the most measurement modes of any multifunctional life science reader.  
The Omegas Tandem Technology (TT) uses an ultra-fast UV/Vis spectrometer for flexibility in absorbance and high performance filters for detection in fluorescence. There are four readers in the Omega line and they can all be upgraded to the top of the line (POLARstar).
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Features of Omega Series
  • Top and bottom reading  
  • Incubation up to 60°  
  • Varied shaking  
  • Two reagent injectors  
  • Well scanning  
  • Orbital averaging  
  • Powerful MARS evaluation software  

Ultra-Fast UV/Vis Spectrometer - Instantaneously capture (~1 sec/well) any range of wavelengths from 220 to 850 nm at resolutions of 1, 2, 5 or 10 nm. Monochromators are NOT used, so no scanning or calibration is required. Monochromators cannot match the speed or performance of a spectrometer. (Available on FLUOstar, POLARstar and SPECTROstar)  

Improve Absorbance Applications - The Omega combines the flexibility and sensitivity of a classic spectrophotometer with the speed and sample capacity of a microplate reader. Perform applications that were not previously possible on a microplate reader:  

  • Easily quantify DNA and protein (260/280/320) with ONE read in <1 second/well  
  • Measure the optimal detection wavelength for an ELISA because ALL wavelengths are given  
  • Monitor enzymatic reactions without knowing the absorbance wavelength of the product or substrate  
  • Instantaneously detect global changes for cofactors like NADH, NO or NADPH without switching filters  
  • Detect interferants in an assay or samples with a full spectral scan  
  • Quickly scan compound libraries for impurities and reference wavelengths  

Fluorescence Intensity - A high intensity xenon flash lamp and carefully selected PMTs, coupled with high transmission band pass filters, provide a stable, low-noise signal platform for ultra sensitive fluorescence readings. The Omega can accurately read any fluorophore from 220-850 nm, including fluorescently tagged proteins, with low limits of detection. (Available on FLUOstar and POLARstar)  

Fluorescence Polarization - Low mP variation is obtained because a high intensity xenon flash lamp, carefully paired PMTs and high performance filters ensure low variability between measurement channels. (Available on POLARstar)  

Simultaneous Dual Emission Detection - Reading two wavelengths at once decreases read times and increases accuracy. (Available on POLARstar)  

TRF and TR-FRET - Optional TRF reading head allows the Omega to obtain unmatched time-resolved fluorescence performance for a life science reader. The Omega can deliver HTS-like performance at a fraction of HTS prices. (Available on FLUOstar and POLARstar)  

FRET/BRET - Fluorescence or Bioluminescence Resonance Energy Transfer is easy on the Omega using features such as SDE, optimized filters and precise reagent injection. No other mid-range reader has SDE, which allows for fast and precise FRET/BRET data. (Available on FLUOstar and POLARstar)  

Luminescence  Dedicated measurement electronics elevate the   luminescence capabilities of the Omega to match stand alone luminometers. (Available on FLUOstar, POLARstar and LUMIstar)  

SPECTROstar and LUMIstar Omega   Upgradable Absorbance and Luminescence Readers  

The SPECTROstar and LUMIstar are BMG LABTECHs dedicated absorbance and luminescence   microplate readers, respectively, that can be upgraded at any time.  

Ultra-Fast Full Spectrum Absorbance   The SPECTROstar uses a proprietary ultra-fast UV/Vis spectrometer to capture the entire spectrum of each sample (220-850 nm) with a 1, 2, 5 or 10 nm resolution. No need to select wavelengths or guess that the optimal wavelength is selected. Get an entire UV/Vis spectrum for each well in ~1 sec/well, or about 1 minute for a 96 well plate. The UV/Vis spectrometer can perform absorbance assays that were not previously possible on a microplate reader. See page 70 for more information.  

High Performance Luminescence   Dedicated measurement electronics, liquid light guides and adjustable gain make the LUMIstar Omega one of the best luminometers on the market. Couple this with the Omegas advanced features (slow and fast kinetics, onboard reagent injectors, variable shaking and top and bottom reading) and the LUMIstar can easily perform all Flash and Glow luminescence assays.  

FLUOstar and POLARstar Omega   Multidetection Readers for the Life Science Laboratory  

The FLUOstar and POLARstar Omegas are the first multidetection microplate reader with a true UV/Vis spectrometer for absorbance. Their innovative design and technology allow for great flexibility and sensitivity in all reading modes.  

Fluorescence Intensity (Including FRET)   A high intensity xenon flash lamp, carefully selected PMTs, high transmission band pass filters, and low light blocking optics provide a low-noise, stable signal platform for ultra sensitive fluorescence readings. Fast filter switching and adjustable gain allow for all fluorescence intensity and FRET based assays to be performed with the FLUOstar Omega.  

Multimode with Simultaneous Dual Emission (SDE) Detection   For assays that require detecting two emission wavelengths, SDE permits the Omega to attain faster and more accurate results when compared to plate readers without this capability. Assay measurements that use SDE detection result in faster read times, as well as better Z' values and %. SDE reduces the inherent variability that results from comparing signals from two separate reads.  

Fluorescence Polarization   Carefully paired photomultiplier tubes, a high intensity xenon flash lamp and assay optimized fluorescence filters ensure low variability between measurement channels, thus giving low mP standard deviations. These features make the POLARstar Omega the best performing FP reader in its price range.  

Omega Upgrades   The Omega readers all share the same BMG LABTECH technology platform, allowing them to grow to handle all life science applications. The following technologies can be added at any time:  

  • AlphaScreen®  
  • Fluorescence Intensity  
  • Luminescence (standard on LUMIstar)  
  • Time-resolved fluorescence (TRF) including TR-FRET  
  • Simultaneous Dual Emission for Fluorescence Polarization  
  • UV/Vis Absorbance (LUMIstar only, standard on others)
General Specifications

There are no General Specifications available.

Additional Specifications

Detection Modes: Absorbance - UV/Vis spectral scanning or standard  
Measurement Modes:   
- Endpoint and kinetic measurements  
- Well scanning  
Light Source: High-energy xenon flash lamp  
Injector: Up to two  
Dispense Volume Range: Up to four different volumes, between 5 and 350 µL in 0.5 µL increments  
Detector: Spectrometer with CCD  
Spectral range: 220 to 850 nm  
Spectrum Capture Time: <1 s/well  
Selectable spectral resolution: 1 nm, 2 nm, 5 nm, 10 nm  
OD range: 0 to 4 OD  
Accuracy: < 1% at 2 OD  
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD  
Dynamic range: ± 0.000 - 4.000 OD  
Reproducibility: ± 0.005 OD for 0 - 2 OD range  
Microplate Formats: Up to 384-well  
Reading Speed: Dependent on test definition  
Temperature Control: Incubation range from ambient +5°C to 45°C (option: 60°C)  
Temperature stability: 0.2°C  
Temperature gradient: < 0.5°C  
Shaking: Linear, orbital, and double-orbital; adjustable time and amplitude  
Stacker II: For automatic plate handling a stacker is available with magazines  
for up to 50 plates (6-384 well plate formats) and barcode reader  
with flexible barcode handling

Series Modals


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