BMG LABTECH - Omega Series
UV/Vis Absorbance Spectrophotometer Microplate Reader; complete spectrum within 1 second
The Omegas Tandem Technology (TT) uses an ultra-fast UV/Vis spectrometer for flexibility in absorbance and high performance filters for detection in fluorescence. There are four readers in the Omega line and they can all be upgraded to the top of the line (POLARstar).
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Features of Omega Series
- Top and bottom reading
- Incubation up to 60°
- Varied shaking
- Two reagent injectors
- Well scanning
- Orbital averaging
- Powerful MARS evaluation software
Ultra-Fast UV/Vis Spectrometer - Instantaneously capture (~1 sec/well) any range of wavelengths from 220 to 850 nm at resolutions of 1, 2, 5 or 10 nm. Monochromators are NOT used, so no scanning or calibration is required. Monochromators cannot match the speed or performance of a spectrometer. (Available on FLUOstar, POLARstar and SPECTROstar)
Improve Absorbance Applications - The Omega combines the flexibility and sensitivity of a classic spectrophotometer with the speed and sample capacity of a microplate reader. Perform applications that were not previously possible on a microplate reader:
- Easily quantify DNA and protein (260/280/320) with ONE read in <1 second/well
- Measure the optimal detection wavelength for an ELISA because ALL wavelengths are given
- Monitor enzymatic reactions without knowing the absorbance wavelength of the product or substrate
- Instantaneously detect global changes for cofactors like NADH, NO or NADPH without switching filters
- Detect interferants in an assay or samples with a full spectral scan
- Quickly scan compound libraries for impurities and reference wavelengths
Fluorescence Intensity - A high intensity xenon flash lamp and carefully selected PMTs, coupled with high transmission band pass filters, provide a stable, low-noise signal platform for ultra sensitive fluorescence readings. The Omega can accurately read any fluorophore from 220-850 nm, including fluorescently tagged proteins, with low limits of detection. (Available on FLUOstar and POLARstar)
Fluorescence Polarization - Low mP variation is obtained because a high intensity xenon flash lamp, carefully paired PMTs and high performance filters ensure low variability between measurement channels. (Available on POLARstar)
Simultaneous Dual Emission Detection - Reading two wavelengths at once decreases read times and increases accuracy. (Available on POLARstar)
TRF and TR-FRET - Optional TRF reading head allows the Omega to obtain unmatched time-resolved fluorescence performance for a life science reader. The Omega can deliver HTS-like performance at a fraction of HTS prices. (Available on FLUOstar and POLARstar)
FRET/BRET - Fluorescence or Bioluminescence Resonance Energy Transfer is easy on the Omega using features such as SDE, optimized filters and precise reagent injection. No other mid-range reader has SDE, which allows for fast and precise FRET/BRET data. (Available on FLUOstar and POLARstar)
Luminescence Dedicated measurement electronics elevate the luminescence capabilities of the Omega to match stand alone luminometers. (Available on FLUOstar, POLARstar and LUMIstar)
SPECTROstar and LUMIstar Omega Upgradable Absorbance and Luminescence Readers
The SPECTROstar and LUMIstar are BMG LABTECHs dedicated absorbance and luminescence microplate readers, respectively, that can be upgraded at any time.
Ultra-Fast Full Spectrum Absorbance The SPECTROstar uses a proprietary ultra-fast UV/Vis spectrometer to capture the entire spectrum of each sample (220-850 nm) with a 1, 2, 5 or 10 nm resolution. No need to select wavelengths or guess that the optimal wavelength is selected. Get an entire UV/Vis spectrum for each well in ~1 sec/well, or about 1 minute for a 96 well plate. The UV/Vis spectrometer can perform absorbance assays that were not previously possible on a microplate reader. See page 70 for more information.
High Performance Luminescence Dedicated measurement electronics, liquid light guides and adjustable gain make the LUMIstar Omega one of the best luminometers on the market. Couple this with the Omegas advanced features (slow and fast kinetics, onboard reagent injectors, variable shaking and top and bottom reading) and the LUMIstar can easily perform all Flash and Glow luminescence assays.
FLUOstar and POLARstar Omega Multidetection Readers for the Life Science Laboratory
The FLUOstar and POLARstar Omegas are the first multidetection microplate reader with a true UV/Vis spectrometer for absorbance. Their innovative design and technology allow for great flexibility and sensitivity in all reading modes.
Fluorescence Intensity (Including FRET) A high intensity xenon flash lamp, carefully selected PMTs, high transmission band pass filters, and low light blocking optics provide a low-noise, stable signal platform for ultra sensitive fluorescence readings. Fast filter switching and adjustable gain allow for all fluorescence intensity and FRET based assays to be performed with the FLUOstar Omega.
Multimode with Simultaneous Dual Emission (SDE) Detection For assays that require detecting two emission wavelengths, SDE permits the Omega to attain faster and more accurate results when compared to plate readers without this capability. Assay measurements that use SDE detection result in faster read times, as well as better Z' values and %. SDE reduces the inherent variability that results from comparing signals from two separate reads.
Fluorescence Polarization Carefully paired photomultiplier tubes, a high intensity xenon flash lamp and assay optimized fluorescence filters ensure low variability between measurement channels, thus giving low mP standard deviations. These features make the POLARstar Omega the best performing FP reader in its price range.
Omega Upgrades The Omega readers all share the same BMG LABTECH technology platform, allowing them to grow to handle all life science applications. The following technologies can be added at any time:
- AlphaScreen®
- Fluorescence Intensity
- Luminescence (standard on LUMIstar)
- Time-resolved fluorescence (TRF) including TR-FRET
- Simultaneous Dual Emission for Fluorescence Polarization
- UV/Vis Absorbance (LUMIstar only, standard on others)
General Specifications
There are no General Specifications available.
Additional Specifications
Detection Modes: Absorbance - UV/Vis spectral scanning or standard
Measurement Modes:
- Endpoint and kinetic measurements
- Well scanning
Light Source: High-energy xenon flash lamp
Injector: Up to two
Dispense Volume Range: Up to four different volumes, between 5 and 350 µL in 0.5 µL increments
Detector: Spectrometer with CCD
Spectral range: 220 to 850 nm
Spectrum Capture Time: <1 s/well
Selectable spectral resolution: 1 nm, 2 nm, 5 nm, 10 nm
OD range: 0 to 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
Dynamic range: ± 0.000 - 4.000 OD
Reproducibility: ± 0.005 OD for 0 - 2 OD range
Microplate Formats: Up to 384-well
Reading Speed: Dependent on test definition
Temperature Control: Incubation range from ambient +5°C to 45°C (option: 60°C)
Temperature stability: 0.2°C
Temperature gradient: < 0.5°C
Shaking: Linear, orbital, and double-orbital; adjustable time and amplitude
Stacker II: For automatic plate handling a stacker is available with magazines
for up to 50 plates (6-384 well plate formats) and barcode reader
with flexible barcode handling
Series Modals
BMG LABTECH - FLUOstar Omega
[+] View Model Specifications| Depth | 48 cm |
| Detector | Side window photomultiplier tube |
| Shaking | 1 |
| Spectral Range | 240 to 740 nm |
| Height | 30 cm |
| Detection Mode | Mid-level |
| Width | 44 cm |
| Detection Method | Fluorescence Intensity - including FRET, AlphaScreen® / AlphaLISA®, Luminescence (flash and glow) - including BRET, Time-Resolved Fluorescence - including TR-FRET, UV/Vis Absorbance Spectra |
| Shaking Pattern | Linear, orbital, and double-orbital with user-definable time and speed |
| Filters | Excitation and emission filter wheels for 8 filters each |
| Sample Formats | 6 to 1536-well plates, user-definable |
| Lighting | High energy xenon flashlamp |
| Weight | 62 lbs |
| Read Method | Top and bottom reading, Endpoint and Kinetic measurements, Sequential Multi Excitation measurements, Sequential Multi Emission measurements, Ratiometric measurements, Well Scanning |
| Wavelength Range | 220 to 850 nm |
| Scanning Speed | Flying mode: 9 s (96), 16 s (384) |
| Microplate Size | 384-well |
| Software | External |
| Temperature Control | 1 |
| Additional Model Specifications | Sensitivity: - FI: < 0.2 fmol/well Fluorescein - TRF: < 30 amol/well Europium - High-End TRF for Omega: < 3 amol/well Europium - LUM: 20 amol/well ATP; DLReady certified - AlphaScreen®: < 100 amol* (384) - ABS with Spectrometer, Spectral range: 220 - 850 nm Full spectrum captured in < 1 s/well Selectable spectral resolution: 1 nm, 2 nm, 5 nm, 10 nm OD range: 0 to 4 OD Accuracy: < 1% at 2 OD Precision: < 0.5% at 1 OD and < 0.8% at 2 OD Reagent injection: - Up to 2 built-in reagent injectors - Injection at measurement position (6 to 384-well) - Individual injection volumes for each well (3 to 350 µL) - Variable injection speed up to 420 µL / s - Up to four injection events per well - Reagent back flushing Gas vent: System to inject an atmosphere or to pull a vacuum into the reader Software: License-free software package including Reader Control and MARS Data Analysis Software Accessories Stacker: - Mid-Throughput Microplate Handling System - Magazines for up to 50 plates - Continuous loading feature THERMOstar: - Microplate Incubator and Shaker - Shakes and incubates four microplates simultaneously - Incubation up to 56°C in 1°C steps - Heating plates above and below the microplates - Barcode reader Filters: - Optimized for dyes, fluophores and specific assays - Filters for all applications from UV to NIR - Customized filters available upon request Upgrades: - Upgrades to a higher model or to include options such as additional detection modes, reagent injectors, extended temperature control, etc. are available. Please contact your local representative for more information. |
BMG LABTECH - LUMIstar Omega
[+] View Model Specifications| Depth | 48 cm |
| Detector | Side window, current type photomultiplier tube |
| Shaking | 1 |
| Spectral Range | 240 to 740 nm |
| Height | 30 cm |
| Detection Mode | Luminescence |
| Width | 44 cm |
| Detection Method | Luminescence (flash and glow) - including BRET |
| Shaking Pattern | Linear, orbital, and double-orbital with user-definable time and speed |
| Filters | One emission filter wheel for 8 filters |
| Sample Formats | 6 to 384-well plates, user-definable |
| Weight | 55 lbs |
| Read Method | Top and bottom reading, Endpoint and Kinetic measurements, Sequential Multi Emission measurements, Simultaneous Dual Emission measurements, Ratiometric measurements |
| Scanning Speed | Flying mode: 20 s (96), 55 s (384) |
| Microplate Size | 384-well |
| Software | External |
| Temperature Control | 1 |
| Additional Model Specifications | Sensitivity: 20 amol/well ATP; DLReady certified Reagent injection: - Up to 2 built-in reagent injectors - Injection at measurement position (6 to 384-well) - Individual injection volumes for each well (3 to 350 µL) - Variable injection speed up to 420 µL / s - Up to four injection events per well - Reagent back flushing Gas vent: System to inject an atmosphere or to pull a vacuum into the reader Software: License-free software package including Reader Control and MARS Data Analysis software Accessories Stacker: - Mid-Throughput Microplate Handling System - Magazines for up to 50 plates - Continuous loading feature - Barcode reader THERMOstar: - Microplate Incubator and Shaker - Shakes and incubates four microplates simultaneously - Incubation up to 56°C in 1°C steps - Heating plates above and below the microplates Filters: - Optimized for dyes, fluophores and specific assays - Filters for all applications from UV to NIR - Customized filters available upon request Upgrades: - Upgrades to a higher model or to include options such as additional detection modes, reagent injectors, extended temperature control, etc. are available. |
BMG LABTECH - POLARstar Omega
[+] View Model Specifications| Depth | 48 cm |
| Detector | Two matched side window photomultiplier tubes |
| Shaking | 1 |
| Spectral Range | 240 to 740 nm |
| Height | 30 cm |
| Detection Mode | Multi-label |
| Width | 44 cm |
| Detection Method | Fluorescence Intensity - including FRET, Fluorescence Polarization, AlphaScreen® / AlphaLISA®, Luminescence (flash and glow) - including BRET, Time-Resolved Fluorescence - including TR-FRET, UV/Vis Absorbance Spectra |
| Shaking Pattern | Linear, orbital, and double-orbital with user-definable time and speed |
| Filters | Excitation and emission filter wheels for 8 filters each |
| Sample Formats | 6 to 1536-well plates, user-definable |
| Lighting | High energy xenon flashlamp |
| Weight | 62 lbs |
| Read Method | Top and bottom reading, Endpoint and Kinetic measurements, Sequential Multi Excitation measurements, Sequential Multi Emission measurements, Simultaneous Dual Emission measurements, Ratiometric measurements, Well Scanning |
| Wavelength Range | 220 to 850 nm |
| Scanning Speed | Flying mode: 9 s (96), 16 s (384) |
| Microplate Size | 384-well |
| Software | External |
| Temperature Control | 1 |
| Additional Model Specifications | Sensitivity: - FI: < 0.2 fmol/well Fluorescein - FP: < 5 mP SD at 1 nM Fluorescein - TRF: < 30 amol/well Europium - High-End TRF for Omega: < 3 amol/well Europium - LUM: 20 amol/well ATP; DLReady certified - AlphaScreen®: < 100 amol* (384) - ABS with Spectrometer: Spectral range: 220 - 850 nm Full spectrum captured in < 1 s/well Selectable spectral resolution: 1 nm, 2 nm, 5 nm, 10 nm OD range: 0 to 4 OD Accuracy: < 1% at 2 OD Precision: < 0.5% at 1 OD and < 0.8% at 2 OD Reagent injection: - Up to 2 built-in reagent injectors - Injection at measurement position (6 to 384-well) - Individual injection volumes for each well (3 to 350 µL) - Variable injection speed up to 420 µL / s - Up to four injection events per well - Reagent back flushing Gas vent: System to inject an atmosphere or to pull a vacuum into the reader Software: License-free software package including Reader Control and MARS Data Analysis Software Accessories Stacker: - Mid-Throughput Microplate Handling System - Magazines for up to 50 plates - Continuous loading feature THERMOstar: - Microplate Incubator and Shaker - Shakes and incubates four microplates simultaneously - Incubation up to 56°C in 1°C steps - Heating plates above and below the microplates - Barcode reader Filters: - Optimized for dyes, fluophores and specific assays - Filters for all applications from UV to NIR - Customized filters available upon request Upgrades: - Upgrades to include options such as additional detection modes, reagent injectors, extended temperature control, etc. are available. Please contact your local representative for more information. |
BMG LABTECH - SPECTROstar Omega
[+] View Model Specifications| Depth | 48 cm |
| Detector | Spectrometer with CCD |
| Shaking | 1 |
| Spectral Range | 220 to 850 nm |
| Height | 29 cm |
| Detection Mode | Absorbance |
| Width | 44 cm |
| Detection Method | Absorbance - UV/Vis spectral scanning or standard |
| Shaking Pattern | Linear, orbital, and double-orbital; adjustable time and amplitude |
| Sample Formats | Up to 384-well plates |
| Lighting | High-energy xenon flash lamp |
| Weight | 66.1 lbs |
| Read Method | Endpoint and kinetic measurements, Well scanning |
| Scanning Speed | Dependent on test definition |
| Microplate Size | 384-well |
| Software | External |
| Temperature Control | 1 |
| Additional Model Specifications | Injector: Up to two Dispense Volume Range: Up to four different volumes, between 5 and 350 µL in 0.5 µL increments Spectrum Capture Time: <1 s/well Selectable spectral resolution: 1 nm, 2 nm, 5 nm, 10 nm OD range: 0 to 4 OD Accuracy: < 1% at 2 OD Precision: < 0.5% at 1 OD and < 0.8% at 2 OD Dynamic range: ± 0.000 - 4.000 OD Reproducibility: ± 0.005 OD for 0 - 2 OD range Temperature Control: Incubation range from ambient +5°C to 45°C (option: 60°C) Temperature stability: 0.2°C Temperature gradient: < 0.5°C Stacker II: For automatic plate handling a stacker is available with magazines for up to 50 plates (6-384 well plate formats) and barcode reader with flexible barcode handling |