Agilent Technologies - Seahorse XFe96 Analyzer

Manufactured by  Agilent Technologies
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Seahorse XFe96 Analyzers measure OCR and ECAR of live cells in a 96-well plate format. OCR and ECAR rates are key...

Seahorse XFe96 Analyzers measure OCR and ECAR of live cells in a 96-well plate format. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis and these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples.
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Features of Seahorse XFe96 Analyzer
  • Real-time Results- this integrated system reports metabolic rates in just minutes, without sample extraction or labeling. Wave software controls the instrument and performs rate measurements on the fly for same day results.           * Live-Cell Responses – detect responses to substrates, inhibitors, and other compounds in real time via the 4-port injection system and automated mixing while maintaining physiologic temperature (37oC).           * Flexible Assay Design- the 96-well plate format accommodates many conditions in a single run and is best for dose-response studies and compound screening.           * High Sensitivity- analyze as few as 5000 cells per well in the custom 96-well plate. Cell number requirements vary by cell type; consult the Cell Reference Database for details.           * Compatible with a variety of samples due to the precision-controlled heating tray, which can maintain 16-42oC (12oC-20oC above ambient conditions).           * Measure mitochondrial function with the Seahorse XF Cell Mito Stress Test           * Generate a metabolic phenotype within one hour with the Seahorse XF Cell Energy Phenotype Test           * Measure glycolytic rates in live cells with the Seahorse XF Glycolytic Rate Assay           * Quickly determine dependency of cellular energy production on mitochondrial substrates with the Seahorse XF Mito Fuel Flex Test           * Analyze 3D samples such as spheroids and islets with the Seahorse XFe96 Spheroid Plate           * Easily create assay protocols and analyze data with Seahorse Wave software
General Specifications

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Additional Specifications

All Agilent Seahorse XF Analyzers Feature:     

  • Compatibility with both adherent and suspension cells as well as isolated mitochondria and non-mammalian samples.     
  • Ability to perform up to 4 independent injections per well with automatic mixing     
  • Automatic calculation of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).     
  • Simultaneous measurement of OCR and ECAR in the same well     
  • Analytical Sensitivity for small sample sizes     
  • Label-free detection in real time     
  • Windows-compatible desktop analysis software (Wave) for plotting, reporting, analyzing, and exporting your XF data
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