The optical design of the SpectraMax 340PC384 truly mimics a dual-beam spectrophotometer. Each well has its own sample beam and reference detector. The eight-channel system, consisting of eight sample beams and eight reference beams, delivers both superior precision and speed of reading across the microplate.
No interference filters required
The SpectraMax 340PC384 has a monochromator instead of interference filters. Simply select the absorbance maximum of your sample and let the monochromator tune to that exact wavelength. Or scan up to 384 wells at any time to determine the best assay conditions. The wavelength and scanning ranges are from 340-850 nm in 1 nm increments. It is like having 511 filters built-in so you will always have the correct wavelength.
Measure fluid volumes in individual wells
The patented* PathCheck® sensor measures the depth of the liquid in each microplate well. This information can be used to normalize absorbance values to a 1 cm pathlength so they will agree with measurements made in a standard 1 cm cuvette. You can also use this feature to calculate concentrations without a standard curve and expand the dynamic range to 6+ OD. PathCheck can accurately measure liquid volumes down to 5 µl to test the accuracy and precision of liquid handling devices.
Leading microplate data analysis software
SoftMax® Pro microplate data analysis software provides flexibility in experimental design, set-up, analysis and reporting, giving you the opportunity to customize your assays exactly as you require. You can password-protect your data, choose from nine different curve-fitting routines and use default data reduction, or set up your own formulas for analysis. You can even analyze and combine data from different plates.
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Features of SpectraMax 340PC384
- No filters needed
- Easy assay optimization
- Well volume sensor
- Comprehensive data analysis software
- Optical validation
- Monochromator instead of interference filters
- PathCheck® sensor measures the depth of the liquid in each microplate well
- SoftMax® Pro microplate data analysis software included
- Optional SPECTRAtest™ Validation Package
- Kinetic ELISAs/Enzyme Assays
- ß-galactosidase Reporter Gene
- Colorimetric Protein
- NAD(P)H-based assay
|Photometric Range||0 to 4.000 OD|
|Wavelength Range||340 to 850 nm|
|Scanning Speed||Endpoint: 96 wells in 9 seconds; 384 wells in 29 seconds. Minimum kinetic interval: 96 wells in 9 seconds; 384 wells in 29 seconds.|
|Wavelength Selection||Monochromator, tunable in 1-nm increments|
Photometric linearity (405 nm): 0 to 3.000 OD
Photometric accuracy (340-850 nm): < or = 0.006 OD ± 1.0%, 0-2 OD
Wavelength accuracy: <± 1.0 nm
Photometric precision: < or = 0.003 OD ± 1.0%, 0-2 OD
Wavelength repeatability: ± 0.2 nm
PathCheck measurement error: < 5% compared to a 1 cm cuvette