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BMG LABTECH - NOVOstar

Manufactured by  BMG LABTECH
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In the past, cell-based assays required the use of expensive HTS equipment, which lead to limited accessibility for...

In the past, cell-based assays required the use of expensive HTS equipment, which lead to limited accessibility for scientists in the assay development stage. In order to reduce this bottleneck, BMG LABTECH developed the NOVOstar, an exciting microplate reader for screening a large number of compounds in fast kinetic, cell-based assays and research development. The NOVOstar is a compact microplate reader offering five detection modes in a unique combination with an integrated pipettor system for automated assembly and instantaneous reading of assays in microplate formats up to 384-well.  
     
  1. Fluorescence Polarization  
  2. Fluorescence Intensity - including FRET and multilable applications  
  3. Time-Resolved Fluorescence - including DELFIA®  
  4. Absorbance - UV/Vis with path length correction  
  5. High-Performance Luminescence (flash and glow) - DLReadyTM certified  
    Unlike a conventional microplate reader, the NOVOstar has a dual microplate carrier that allows the user to designate one as a reagent plate and the other as a measurement plate.     An integrated transfer pipettor delivers compounds from the reagent plate or from one of the three reagent stations to the measurement plate allowing the user to prepare wells   or start kinetic events. Using this system, up to 384 compounds can be screened in a signalling assay - virtually impossible to do using a conventional plate reader.     Additionally, two onboard reagent injectors can deliver variable volumes of reagent to wells giving you a wide range of liquid handling options, combined with the measurement abilities of a conventional multifunctional microplate reader.
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Features of NOVOstar

High performance luminescence      

The NOVOstar has been designed with two dedicated measurement systems, one for fluorescence and one for luminescence. Eliminating the need to make a choice between       a dedicated fluorometer and luminometer. The NOVOstar offers exceptional luminescence performance in a single instrument package that easily fulfils Promega’s stringent       DLReadyTM (dual luciferase validation) criteria in 96- and 384-well plate formats.      

Optimized for kinetic assays      

The NOVOstar is optimized to monitor fast kinetic events, such as calcium flux. These types of assays can easily be triggered using the transfer pipettoror one of the two built-in injectors. Reagent addition and measurements can be undertaken concurrently to ensure even the fastest kinetics are captured. Data can be collected at different rates within the same experiment. For example, fast kinetic flux assays typically produce the bulk of their data in the first few seconds after injection. Using the multiple kinetic window feature you can collect data during this critical time with high resolution,       as fast as 50 measurement points per second. After the peak you can reduce the capture rate to 1 read every few seconds thus reducing your dataset and analysis time.      

Advanced liquid handling      

The NOVOstar includes a unique integrated transfer pipettor and two additional syringe injectors. All three liquid dispensing devices have direct access to the measurement position, allowing plate reading before, during, and after addition of liquid samples. The transfer pipettor is able to aspirate, dispense, and mix precise volumes of fluids from the reagent plate or from three reagent stations. The delivery volume is adjustable for each well from 0.5 to 100 µL, allowing you to produce dilution schemes, concentration ranges or standard curves. The injectors can be used for adding standard       reagents to selected wells in the measurement plate.      

Multichromatic detection modes      

Sixteen built-in fi lters and fast filter switching allow sequential dual excitation/emission measurements. This feature is important for functional cellular assays using ratiometric ion indicators or voltage-sensitive FRET probes. The NOVOstar       with the fluorescence polarization option can even collect data at two emission wavelengths at the same time. The simultaneous dual emission detection technology together with real-time ratio calculation substantially reduce plate read times and increase performance of FRET based assays.      

Control and evaluation software      

The WindowsTM based PC software provides an extensive range of options for assay design and data evaluation. Control software allows users to define instrument parameters       including injection timing, mixing, shaking, pump speed, etc. During a plate measurement, the current state feature can be used to observe the progress of kinetic reactions in all wells.      

The evaluation software is based on powerful ExcelTM macros. Worksheets display raw data, calculations, signal plots, and standard curves. Powerful evaluation sheets are       pre-programmed to perform calculations such as %, ratios, curve fitting, FP, anisotrophy, dilution factors, etc. In addition, you can create your own workbooks for specific data evaluation.      

Applications      

  • Ca2+ uptake      
  • Membrane potential studies (Application Note 123)      
  • Intracellular pH      
  • Intracellular cAMP, e.g. FRET based assay with CFP      
  • Measurement of intracellular ionic changes      
  • Cell proliferation assay, e.g. with Alamar Blue      
  • Fluorescent reporter gene assays, e.g. GFP, YFP/CFP FRET-based assay      
  • FRET-based assay, e.g. SUMO protease activity with YFP and enhanced CFP as donor-acceptor pair FRET-based voltage sensor probes      
  • Dual Luciferase Reporter Assay, e.g. determination of siRNA silencing efficiency      
  • Cell adhesion assay, e.g. with calcein AM      
  • Enzyme activity, e.g. 2-OG hydroxylase enzymes (Application Note 132), histone deacetylase inhibition in cancer research      
  • ELISA
General Specifications
Depth 53 cm
DetectorSide window photomultiplier tube
Shaking1
Spectral Range240 to 740 nm
Height30 cm
Detection ModeMid-level
Width 78 cm
Detection MethodFluorescence Intensity - including FRET, Fluorescence Polarization, Luminescence (flash and glow), Time-Resolved Fluorescence - including DELFIA®, UV/Vis absorbance
Shaking PatternLinear, orbital, and double-orbital with user-definable time and speed
FiltersExcitation and emission filter wheels for 8 filters each
Sample Formats6 to 384-well plates, user-definable
LightingHigh energy xenon flashlamp
Weight121 lbs
Read MethodTop and bottom reading, Endpoint and Kinetic measurements, Sequential Multi Excitation measurements, Sequential Multi Emission measurements, Simultaneous Dual Emission measurements, Ratiometric measurements, Well Scanning
Scanning SpeedFlying mode: 14 s (96), 27 (384)
Microplate Size384-well
SoftwareExternal
Temperature Control 1
Additional Specifications

Microplate carrier: Accommodates two plates: one reagent plate and one      
measurement plate      
Sensitivity:      
FI: < 1 fmol / well Fluorescein      
FP: < 5 mP standard deviation at 1 nM fl uorescein      
TRF: < 70 amol/well Europium      
LUM: < 30 amol/well ATP; DLReady certified      
ABS: Dynamic range: ±0.000 - 4.000 OD; reproducibility: ±0.010 OD for 0 - 2 OD range      
Reagent injection:      
Up to 2 built-in reagent injectors      
Injection at measurement position (6 to 384-well)      
Individual injection volumes for each well (3 to 350 µL)      
Variable injection speed up to 420 µL / s      
Up to four injection events per well      
Reagent back flushing      
Pipettor system:      
Injection volume: 0.5 µl - 100 µl      
Individual injection volumes for each well      
Variable injection speed      
Plate-to-plate transfer + 3 reagent stations + washing station      
Gas vent: System to inject an atmosphere or to pull a vacuum into the reader      
Software: License-free software package including Reader Control and MARS      
Data Analysis Software      
      
Accessories      
THERMOstar: Microplate Incubator and Shaker      
Filters:      
Optimized for dyes, fluorophores and specific assays      
Filters for all applications from UV to NIR      
Customized filters available upon request      
Upgrades: Upgrades to include options such as additional detection modes,      
reagent injectors, etc. are available. Please contact your local      
representative for more information.

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