BMG LABTECH - BMG LABTECH - PHERAstar FSX
BMG Labtech's latest, most sensitive microplate reader.
The PHERAstar® FSX is our latest, most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. Thanks to the unique Optic Module system, Simultaneous Dual Emission, UV/vis spectrometer, and dedicated AlphaScreen® and TRF lasers, the PHERAstar FSX will perform your assay with ease and speed, even in 3456-well microplates.
No longer worry about installing filters and dichroics: the PHERAstar FSX comes with easy-to-handle, assay-optimized Optic Modules which contain all application-specific filters, mirrors, dichroics and/or polarizers, and are automatically recognized by the plate reader. In addition, the PHERAstar FSX is equipped with four matched and assay-optimized photomultiplier tubes (PMTs) which are automatically selected for the relevant detection mode. You can also measure assays with two emission wavelengths at the same time thanks to the Simultaneous Dual Emission.
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Features of BMG LABTECH - PHERAstar FSX
Accessories
- Filters and Optic Modules: standard filters from 240 - 850 nm, filters and Optic Modules optimised for specific applications, and bespoke solutions are available
- Microplate Stacker: optimises microplate handling and increases throughput by providing rapid loading, unloading, restacking, and continuous feeding for up to 50 microplates
- LVis Plate: microplate for low-volume DNA, RNA and protein quantification. It includes 16 micro-drop (2 µL) wells, a cuvette slot and optional performance testing features
- Evaluation Plate: validates the optical functionality of BMG LABTECH microplate readers for quality control and/or qualification (IQ/OQ) purpose
For HTS automation purposes, the PHERAstar FSX offers improved robotic integration capabilities and multi-user control.
Detection Modes
Fluorescence intensity - incl. FRET
Fluorescence polarization
AlphaScreen®/AlphaLISA®/AlphaPlexTM
Luminescence (flash and glow) - incl. BRET
Time-resolved fluorescence
TR-FRET
UV/Vis absorbance spectra
Measurement Modes
Top and bottom reading
Endpoint and kinetic
Sequential multi-excitation
Sequential multi-emission
Simultaneous Dual Emission
Spectral scanning (absorbance)
Real-time ratiometric measurements
Well scanning
Microplate Formats
Up to 3456-well plates, user-definable LVis Plate with 16 low volume microspots (2 μL)
Microplate Carrier
Robot compatible
Light Sources
High energy xenon flash lamp
Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlexTM
Dedicated laser for TRF and TR-FRET
General Specifications
There are no General Specifications available.
Additional Specifications
Two matched pairs of photomultiplier tubes (PMTs), optimized for different detection modes
CCD spectrometer
Wavelength Selection
Optic Modules: Up to six application-specific modules (includes all filters, dichroics, etc. for an application)
For top and bottom reading
UV/vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec / well
Optical Path Guides
Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics
Z-Adjustment
Automatic focal height adjustment (0.1 mm resolution)
Spectral Range
Filters 230 - 750 nm or 230 - 900 nm for FI, FP
230 - 750 nm for LUM
230 - 900 nm for TRFSpectrometer 220 - 1000 nm for ABS Sensitivity
FI
(top)< 0.15 pM FITC (384sv black, 20 μL)
< 0.5 pM FITC (1536 black, 8 μL)FI
(bottom)< 1.0 pM FITC (384g black, 50 μL) FP < 0.5 mP SD at 1 nM FITC (384sv black, 20 μL)
< 1.5 mP SD at 1 nM FITC (1536 black, 8 μL)TRF
with laser< 5 fM europium (384, 80 μL)
< 15 fM europium (1536, 8 μL)HTRF®
(black and white microplates)Reader Control Kit (Eu) after 18h (384sv, 20 μL)
Delta F > 1100 % (High Calibrator)
Delta F > 25 % (Low Calibrator)LUM < 0.4 pM (< 8 amol/well ATP, 384sv white, 20 μL)
Dynamic Range: 9 decadesAlphaScreen®
with laser< 5 pM (< 100 amol/well P-Tyr-100, 384sv white, 20 μL) ABS
with spectrometerFull spectrum captured in < 1 s / well
Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 - 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 ODRead Times
Flying mode
(1 flash)14 s (384)
27 s (1536)10 flashes 38 s (384)
1 min 52 s (1536)50 flashes 1 min 29 s (384)
5 min 18 s (1536)Reagent Injection
Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL, optionally up to 2 mL)
Variable injection speed up to 420 µL / s
Up to four injection events per well
Reagent back flushing
Linear, orbital, and double-orbital with user-definable time and speed
Up to three integrated barcode readers
+5 °C above ambient up to 45 °C
The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.
Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant
Dimensions
Width: 45 cm, depth: 51 cm, height: 47 cm; weight: 49 kg