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BMG LABTECH - BMG LABTECH - PHERAstar FSX

Manufactured by  BMG LABTECH
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BMG Labtech's latest, most sensitive microplate reader. 

The PHERAstar® FSX is our latest, most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. Thanks to the unique Optic Module system, Simultaneous Dual Emission, UV/vis spectrometer, and dedicated AlphaScreen® and TRF lasers, the PHERAstar FSX will perform your assay with ease and speed, even in 3456-well microplates.

No longer worry about installing filters and dichroics: the PHERAstar FSX comes with easy-to-handle, assay-optimized Optic Modules which contain all application-specific filters, mirrors, dichroics and/or polarizers, and are automatically recognized by the plate reader. In addition, the PHERAstar FSX is equipped with four matched and assay-optimized photomultiplier tubes (PMTs) which are automatically selected for the relevant detection mode. You can also measure assays with two emission wavelengths at the same time thanks to the Simultaneous Dual Emission.

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Features of BMG LABTECH - PHERAstar FSX

Accessories

  • Filters and Optic Modules: standard filters from 240 - 850 nm, filters and Optic Modules optimised for specific applications, and bespoke solutions are available
  • Microplate Stacker: optimises microplate handling and increases throughput by providing rapid loading, unloading, restacking, and continuous feeding for up to 50 microplates
  • LVis Plate: microplate for low-volume DNA, RNA and protein quantification. It includes 16 micro-drop (2 µL) wells, a cuvette slot and optional performance testing features
  • Evaluation Plate: validates the optical functionality of BMG LABTECH microplate readers for quality control and/or qualification (IQ/OQ) purpose

For HTS automation purposes, the PHERAstar FSX offers improved robotic integration capabilities and multi-user control.

Detection Modes

Fluorescence intensity - incl. FRET

Fluorescence polarization

AlphaScreen®/AlphaLISA®/AlphaPlexTM

Luminescence (flash and glow) - incl. BRET

Time-resolved fluorescence

TR-FRET

UV/Vis absorbance spectra

Measurement Modes

Top and bottom reading

Endpoint and kinetic

Sequential multi-excitation

Sequential multi-emission

Simultaneous Dual Emission

Spectral scanning (absorbance)

Real-time ratiometric measurements

Well scanning

Microplate Formats

Up to 3456-well plates, user-definable LVis Plate with 16 low volume microspots (2 μL)

Microplate Carrier

Robot compatible

Light Sources

High energy xenon flash lamp

Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlexTM

Dedicated laser for TRF and TR-FRET

General Specifications

There are no General Specifications available.

Additional Specifications

Two matched pairs of photomultiplier tubes (PMTs), optimized for different detection modes
CCD spectrometer

  • Wavelength Selection

    Optic Modules: Up to six application-specific modules (includes all filters, dichroics, etc. for an application) 

    For top and bottom reading

    UV/vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec / well

  • Optical Path Guides

    Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics

  • Z-Adjustment

    Automatic focal height adjustment (0.1 mm resolution)

  • Spectral Range

    Filters230 - 750 nm or 230 - 900 nm for FI, FP
    230 - 750 nm for LUM
    230 - 900 nm for TRF
    Spectrometer220 - 1000 nm for ABS
  • Sensitivity

    FI
    (top)
    < 0.15 pM FITC (384sv black, 20 μL)
    < 0.5 pM FITC (1536 black, 8 μL)
    FI
    (bottom)
    < 1.0 pM FITC (384g black, 50 μL)
    FP< 0.5 mP SD at 1 nM FITC (384sv black, 20 μL)
    < 1.5 mP SD at 1 nM FITC (1536 black, 8 μL) 
    TRF
    with laser
    < 5 fM europium (384, 80 μL)
    < 15 fM europium (1536, 8 μL)  
    HTRF®
    (black and white microplates)
    Reader Control Kit (Eu) after 18h (384sv, 20 μL)
    Delta F > 1100 % (High Calibrator)
    Delta F > 25 % (Low Calibrator)
    LUM< 0.4 pM (< 8 amol/well ATP, 384sv white, 20 μL)
    Dynamic Range: 9 decades 
    AlphaScreen®
    with laser
    < 5 pM (< 100 amol/well P-Tyr-100, 384sv white, 20 μL)
    ABS
    with spectrometer
    Full spectrum captured in < 1 s / well
    Selectable spectral resolution: 1, 2, 5, and 10 nm
    OD range: 0 - 4 OD
    Accuracy: < 1% at 2 OD
    Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
  • Read Times

    Flying mode
    (1 flash) 

    14 s (384)
    27 s (1536) 

    10 flashes38 s (384)
    1 min 52 s (1536) 
    50 flashes1 min 29 s (384)
    5 min 18 s (1536) 
  • Reagent Injection

    Up to 2 built-in reagent injectors

    Injection at measurement position (6 to 384-well)

    Individual injection volumes for each well (3 to 500 µL, optionally up to 2 mL)

    Variable injection speed up to 420 µL / s

    Up to four injection events per well

    Reagent back flushing

Shaking

Linear, orbital, and double-orbital with user-definable time and speed


Integrated Barcode Reader

Up to three integrated barcode readers


Incubation

+5 °C above ambient up to 45 °C

The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.


Software

Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant

  • Dimensions

    Width: 45 cm, depth: 51 cm, height: 47 cm; weight: 49 kg

     

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