BMG LABTECH - BMG Labtech - FLUOSTAR OMEGA
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FLUOSTAR Omega multi-mode microplate reader
The FLUOstar® Omega is a multi-mode microplate reader with six detection modes. It utilizes an ultra-fast UV/vis spectrometeror filters for absorbance as well as highly sensitive filters for all other detection modes. The FLUOstar Omega is the ideal plate reader for life science applications.
The FLUOstar Omega is a versatile multi-mode microplate reader with six detection modes: fluorescence intensity (including FRET), luminescence (including BRET), UV/vis absorbance, time-resolved fluorescence, TR-FRET, and AlphaScreen®/AlphaLISA®.
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Features of BMG Labtech - FLUOSTAR OMEGA
- High-speed absorbance measurements
- Measurements in up to 384-well plates
- Top and bottom reading
- Incubation up to 45 or 65 °C
- User-defined shaking
- Well scanning with up to 900 data points per well
- High-precision injectors with simultaneous reagent injection and detection
- Low-volume measurements
General Specifications
There are no General Specifications available.
Additional Specifications
Detection Modes
Fluorescence intensity - incl. FRET
AlphaScreen®/AlphaLISA®
Luminescence (flash and glow) - incl. BRET
Time-resolved fluorescence
TR-FRET
UV/Vis absorbance spectra
Measurement Modes
Top and bottom reading
Endpoint and kinetic
Sequential multi excitation
Sequential multi emission
Spectral scanning (absorbance)
Ratiometric measurements
Well scanning
Microplate Formats
6 to 384-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)
Microplate Carrier
robot compatible
Light Sources
High energy xenon flash lamp
Detectors
Low noise photomultiplier tube
CCD spectrometer
Wavelength Selection
Optical filters: ex and em wheels for 8 filters each
UV/vis absorbance spectrometer: full spectra or 8 distinct wavelengths in < 1 sec / well
Optical Filters
Excitation and emission filter wheels for 8 filters each
Optical Path Guides
Top: liquid-filled light guides
Bottom: fiber optics
Spectral Range
Filters
240 - 740 nm or 240 - 900 nm for FI, FP, TRF
240 - 740 nm for LUM
Spectrometer
220 - 1000 nm for ABS
Sensitivity
FI < 0.2 fmol/well FITC
TRF < 30 amol/well europium
High-End TRF for Omega < 3 amol/well europium
LUM < 20 amol/well ATP
AlphaScreen®
< 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL)
ABS
with spectrometer
Full spectrum captured in < 1 s/well
Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 to 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
Read Times
Flying mode (1 flash)
9 s (96), 16 s (384)
Reagent Injection
Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL)
Variable injection speed up to 420 µL / s
Up to four injection events per well
Reagent back flushing
Shaking
Linear, orbital, and double-orbital with user-definable time and speed
Purge Gas Vent
System to inject an atmosphere or to pull a vacuum into the reader
Incubation
+4 °C above ambient up to 45 °C or 65 °C
The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.
Software
Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant
Dimensions
Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg
- Atmospheric Control Unit (ACU): provides the ideal environment for cell-based assays by regulating O2 and CO2 gas levels in the microplate reader
- Filters and Optic Modules: standard filters from 240 - 850 nm, filters and Optic Modules optimised for specific applications, and bespoke solutions are available
- Microplate Stacker: optimises microplate handling and increases throughput by providing rapid loading, unloading, restacking, and continuous feeding for up to 50 microplates
- LVis Plate: microplate for low-volume DNA, RNA and protein quantification. It includes 16 micro-drop (2 µL) wells, a cuvette slot and optional performance testing features
- Evaluation Plate: validates the optical functionality of BMG LABTECH microplate readers for quality control and/or qualification (IQ/OQ) purposes