• High-resolution in both dimensions by exploiting the wide-ranging ionic and hydrophobic structure of peptides    
• Better protein identifications, quantification, and sequence coverage    
• Improved separation, method generation wizards, and enhanced data algorithms     

     [u]High Resolution Separations in Both Dimensions[/u]          When ion exchange is used as the first separation dimension, poor resolution and bleed between fractions could occur. Using a highly resolving support in the first step provides you with better information in the last step. A reversed phase support at pH 10, followed by a sub-2-micron separation at lower pH, useful, orthogonal separations that yield high quality data is obtained.          [img]http://www.waters.com/webassets/cms/category/media/detail_page_images/nanoACQUITY_2D_detail_1.jpg[/img]     [i]Gilar M. ET AL. J.Sep.Sci. 2005,28,1694-1703.[/i]          A salt-free approach was used to change the selectivity of the separation. A new selectivity was achieved and maintained chromatographic resolution whole using MS-friendly, volatile mobile phase modifiers.          [u]Secure Identifications for Best-In-Class Reproducibility[/u]          An increased number of secure identifications is shown as a function of 2D-LC resolution. Both reproducible and incremental identifications of proteins based on 2D-LC can be seen.          [img]http://www.waters.com/webassets/cms/category/media/detail_page_images/nanoACQUITY_2D_detail_4.jpg[/img]          [u]Increased Resolution Equals Better Protein Identifications[/u]          In this separation, the power of 2D-LC for resolution of complex proteomic samples is demonstrated by this tryptic digest of E. coli. Using nanoACQUITY® in one dimension, 0.5 µg were loaded on-column to resolve and identify 365 proteins from 2874 peptides. Identification of 7661 and 9415 peptides from the 5-step and 10-step separations respectively were obtained by adding 2.5 µg to the 2D-LC System.          The 2D-LC enabled         

    
• Identification of twice as many proteins more securely based    
• Recovery of more peptides    
• Greater protein coverage with high confidence in results    

     [img]http://www.waters.com/webassets/cms/category/media/detail_page_images/nanoACQUITY_2D_detail_3.jpg[/img]          [u]nanoACQUITY 2D-LC Configuration[/u]          To load the sample, inject from the sample manager onto an RP column packed with 3 µm particles (RP1). RP1 provides loading capacity without sacrificing resolution. Then, elute fractions from RP1 to the trapping column and on to RP2, the more highly resolving sub-2-mircon column, for higher peak capacity and better data quality.          The nanoACQUITY UPLC® System is equipped with two valves: one for injection in the sample manager and a second valve in the heating and trapping module (HTM). The entire method is driven from MassLynx™ Application Manager through method generators or wizards.          [img]http://www.waters.com/webassets/cms/category/media/detail_page_images/nanoACQUITY_2D_detail_2.jpg[/img]