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The second-generation SYNAPT™ G2 platform from Waters provides a new dimension of performance to drive your...

The second-generation SYNAPT™ G2 platform from Waters provides a new dimension of performance to drive your scientific discovery like never before. We’ve combined QuanTof™ – breakthrough quantitative Tof technology – and enhanced High Definition MSTM technologies to provide you with intuitive operation, application flexibility, and a totally new level of performance for all your applications.     The MALDI SYNAPT™ G2 HDMS System combines the speed and simplicity of MALDI with ion mobility-based separations and high-resolution mass spectrometry to deliver enhanced specificity for the analysis of the most complex mixtures and challenging discovery applications.     The ion mobility separation capability of the SYNAPT HDMS System enables components previously invisible to traditional MALDI analyses to be revealed, allowing species of interest within complex biological extracts to be separated from isobaric co-eluting compounds, MALDI matrix ions, or background interferences.     Direct profiling of tissue sections by MALDI mass spectrometry using molecular imaging is a powerful procedure to study the spatial distribution of large and small molecules directly from a biological matrix.     The depiction of mass spectral data images as two-dimensional images allows the spatial distribution of molecules to be determined visually. Unlike the time-consuming and costly traditional methods of spatial profiling, such as autoradiography and scintillation counting, radio-labeling is not required. MALDI SYNAPT HDMS uniquely offers HDI™ (High Definition Imaging), which is the combination of MALDI imaging with the separative capability of high efficiency ion mobility separation.     The system is operable in time-of-flight (TOF) mode for exact mass MALDI/MS and MS/MS experiments, and HDMS mode for performing novel ion mobility separations-based investigations.     The exact mass MS and MS/MS capabilities of the MALDI SYNAPT HDMS System enable you to produce optimal results in your proteomic, pharmaceutical, and imaging experiments; the enhanced IMS resolving power enables the increased separation of complex mixtures and previously unresolvable isobaric ions.
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Novel Capabilities and Ultimate Productivity    

Second-generation MALDI SYNAPT™ G2 HDMS™ is a flexible research platform that can provide the ultimate in quantitative and qualitative performance with exact mass UPLC®/MS/MS in TOF mode and the ability expand your analytical capabilities beyond conventional mass spectrometry, combining MALDI with high-performance exact mass MS/MS and high-efficiency ion mobility separations.    


  • Triwave™ Technology: provides enhanced ion mobility separation power and access to its unique benefits in conformational studies, analysis of isomers, reduces sample complexity, increases peak capacity, and enhances information from fragmentation studies.    
  • Facile determination of molecular collision cross-section (shape).    
  • Time aligned parallel (TAP) fragmentation: provides comprehensive structural (MS/MS/MS) information in a single experiment.    
  • DriftScope™ Mobility Environment Software (v. 2.1): for interactive visualization and manipulation of multidimensional data.    
  • HDI™ (High Definition Imaging): the extra dimension of separation provided by the MALDI SYNAPT HDMS System can be used to produce images without interference from background ions of similar mass, leading to more precise localization of a drug or metabolite of interest. Ions are separated using ion mobility (IMS) prior to mass analysis.    

ToF mode:    

  • IntelliStart™ Technology: for simplified system setup and automated system control.    
  • ACQUITY UPLC® Technology: for the highest chromatographic resolution, speed, and sensitivity.    
  • QuanTof™ Technology: ensures you’ll capture the most complete and informative exact mass data in UPLC® timeframes, complemented by UPLC/MSE capability to capture all of the data all of the time.    
  • MassLynx™ Informatics: combines the power of exact mass data and a built-in understanding of chemistry to deliver simple and rapid data interpretation and compound identification.    

Triwave Technology    

Triwave™ Technology is the enabling technology behind the MALDI SYNAPT™ G2 HDMS™ System. It uses three T-Wave™* ion guides that trap ions, separate them by their ion mobility, and transfer them to the orthogonal acceleration time-of-flight analyzer for high-resolution mass analysis. The trap and transfer regions can also be used for fragmentation analyses.    

The Triwave Technology’s innovative configuration overcomes the major performance drawbacks of conventional ion mobility spectrometry (IMS) by ensuring that IMS does not compromise sensitivity.    

The second-generation Triwave technology of SYNAPT G2 provides higher IM resolution through the increased operating pressure and length of the IMS T-Wave , while maintaining high transmission efficiency (with the use of Helium-filled entry cell).    

* The travelling wave device described here is similar to that described by Kirchner in US Patent 5,206,506 (1993).    

Atmospheric Solids Analysis Prove    

The Atmospheric Solids Analysis Probe (ASAP) is a powerful tool for the rapid direct analysis of volatile and semi-volatile, solid and liquid samples using atmospheric pressure ionization.    

The ASAP technique utilizes the heated nitrogen desolvation gas to vaporize the sample and a corona discharge for sample ionization. This allows low polarity compounds not amenable to ESI, APcI, and APPI to be ionized with a high degree of sensitivity. Furthermore complex mixtures can be analyzed without the need for any sample preparation.    

The AtmosphericSolids Analysis Probe is readily fitted to a standard API source by replacing either the ESI or APcI probe assembly. A corona discharge pin also requires fitting. The close proximity of the sample, when loaded onto a glass capillary tube, to the point of ionization and the MS inlet, improves sensitivity whilst also ensuring safety due to the enclosed source housing.    

Instruments are operated in combined Electrospray/APcI mode (ESCi®). This provides for the acquisition of both analyte data in APcI mode and reference data in electrospray mode, enabling exact mass measurements to be produced.    

The probe is designed to have both fixed & removable sections to allow rapid sample introduction without removal of the complete assembly ensuring stable source conditions.    

DriftScope Mobility Environment Software (v. 2.1)    

Unlock the full power of SYNAPT HDMS    

DriftScope™ Mobility Environment Software (v. 2.1) incorporates new technologies to enable comprehensive retrieval of information contained within HDMS™ (ion mobility) datasets while enabling easier data interpretation (for users of all experience levels) through a series of data visualization and data export enhancements:    

Unique capabilities    

  • Enables comparison of measured and theoretical collision cross-sections (shape).    
  • Confident detection of partially (IM) separated compounds (e.g. Isomers or Conformers).    
  • Visualize new molecular trends in complex mixtures (e.g. crude oil, PEG, MALDI imaging).    
  • More efficient characterization of compounds with novel IMS-based fragmentation studies.     

Unique technology    

  • Powerful and automated peak detection (novel APEX 3D and 4D technology) comprehensively reveals all components within any HDMS data (e.g. IMS/MS, LC/IMS/MS), increases apparent specificity of each analysis, eliminates subdrjectivity in data interpretation, and provides highly reproducible results across analyses/users.    
  • IMS Calibration and CCS determination tools provides facile determination of collision cross-sections from experimental data and known 3D structures.    
  • Intuitive visualization tools enable easier and more intuitive data interpretation and provides convenient comparison of peaks and supporting raw data    
  • Peak export functionality facilitates data transfer to downstream processing routines and streamlines data processing workflows e.g. (export of detected peaks directly to Massfragment Software and Elemental composition calculator).    


DriftScope Mobility Environment Software (v. 2.1) provides automated peak detection from 2D (chromatograms and mass spectra) or 3D graphical plots. The axes are interchangeable axes; each pixel represents and ion and its color intensity. The peaks are represented as colored (green) dots and sticks. The data shown is from the electrospray (infusion-based) IMS-MS analysis of a Polyethylene Glycol (PEG) sample.    

Flexibility and Versatility    

The simplicity engineered into the source design of the MALDI SYNAPT™ G2 HDMS™ enables you to quickly and easily switch to and from the API mode of operation.    

The MALDI mode features:    

  • A unique motorized engagement system for fast and easy source changeover.    
  • An attenuable 200 Hz solid state UV laser.    
  • Real-time analysis monitoring via highly magnified digital display optics.    

In API Mode, Waters SYNAPT G2 MS Systems can be configured with ACQUITY UPLC® or nanoACQUITY UPLC® systems and MassLynx™ Application Managers to provide enhanced quality and productivity to a variety of key applications in life science and therapeutic discovery and development.    

For maximum versatility in API mode, Waters source technology enables the widest range of ionization techniques to be utilized universally across a single analytical platform. Providing the highest performance without compromise, the ionization source options are quick and easy to interchange.    

  • ESI/APCI/ESCi®: For the analysis of polar and semi-polar analytes, when coupled with the ACQUITY UPLC® System.    
  • Atmospheric Solids Analysis Probe (ASAP): For the rapid analysis of volatile and semi-volatile solid and liquid samples using atmospheric pressure ionization.    
  • Nano-flow ESI: For the analysis of minute sample amounts when coupled with the nanoACQUITY UPLC® System.    
  • TRIZAIC™ nanoTile™ Technology: Using micro-fabricated nanofluidic UPLC separations, it utilizes sub-2 µm particle chemistries to provide the very best chromatographic performance simply and without compromise.    
  • Atmospheric Pressure GC: Enables rapid switching from LC/MS/MS to GC/MS/MS applications on a single instrument platform. For the analysis of volatile and semi-volatile compounds of low and intermediate polarity.
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