Sequence coverage in proteomics studies can be increased by employing both MALDI and Electrospray Ionization. The MALDI SYNAPT™ MS enables vacuum MALDI MS and MS/MS analyses to be carried out easily with high resolution, sensitivity, and mass measurement accuracy.          The high quality MALDI/MS/MS data produced by the SYNAPT MS enables:    

    
• An increase in protein identifications and protein sequence coverage    
• Elimination of false positive protein identifications apparent in nominal mass data    
• Significant improvement of de novo sequencing compared to post-source decay (PSD) techniques    

     Related Applications          Direct profiling of tissue sections by MALDI mass spectrometry and molecular imaging is a powerful complement to existing methods of biomarker discovery.          In API Mode, Waters SYNAPT™ MS Systems can be configured with ACQUITY UPLC® or nanoACQUITY UPLC® and MassLynx™ Application Managers to provide enhanced quality and productivity to a variety of key applications in life science and therapeutic discovery and development.          Proteomics research - Waters High Definition Proteomics Systems enable a new era in biomedical research by leveraging high bandwidth UPLC/MSE data acquisition with statistically rigorous, ‘chemically intelligent’ informatics for more secure and comprehensive identification and quantification.          Biopharmaceutical characterization - BiopharmaLynx™ Application Manager simplifies the task of biotherapeutic characterization and quality control, by automating the processing and comparison of peptide maps, and intact mass analysis of high molecular weight proteins and antibodies.          Pharmaceutical R&D - Metabonomics, metabolite identificaiton, impurity profiling, and tissue imaging. Waters chemically intelligent system solutions deliver more efficient evaluation of candidates in the therapeutic development and monitoring quality in product manufacturing. Structural characterization is achieved via an intelligent and automated structure-driven workflow, providing enhanced confidence and significantly improving throughput.          [img]http://www.waters.com/webassets/cms/category/media/content_box_images/SYNAPT_MS_detail_1.jpg[/img]          Upgradeability          Future proof your laboratory. SYNAPT™ MS Systems are fully upgradeable.          The true power of a scientific breakthrough is its impact on the world outside the laboratory walls. For laboratories to rise to the world’s greatest challenges, scientists must think beyond the science performed in organizations today.          SYNAPT MS systems are next generation quadrupole oa-TOF platforms which can be transformed on-site to incorporate full High Definition MS functionality, allowing you to analyze samples that are differentiated by size, shape and charge as well as mass to deliver increased specificity and sample definition beyond that achievable by conventional MS.          [img]http://www.waters.com/webassets/cms/category/media/content_box_images/SYNAPT_MS_detail_2.jpg[/img]          SYNAPT HDMS Systems: HDMS Mode and TOF Mode: Access unique benefits of high-efficiency IMS and tandem MS to carry out conformational studies, reduce spectral complexity/background interferences, and retrieve more information from fragmentation studies.          MALDI and API: Provide the ultimate flexibility in MS mode and HDMS mode for high-efficiency IMS separation of ions from MALDI and API.          SYNAPT MS Systems: Access to new levels of productivity with application specific system solutions.          Interchangeable Operation Modes          The source design of the MALDI SYNAPT™ HDMS™ enables you to quickly and easily switch to and from the API mode of operation.          MALDI Mode features:    

    
• A unique motorized engagement system for fast and easy source changeover    
• An attenuable 200 Hz solid state UV laser    
• Real-time analysis monitoring via highly magnified digital display optics    

     In API Mode Waters SYNAPT MS Systems can be configured with ACQUITY UPLC®, or nanoACQUITY UPLC® and MassLynx™ Application Managers to provide enhanced quality and productivity to a variety of key applications in life science and therapeutic discovery and development.          Enabling Technology          SYNAPT™ MS Systems provide you with a flexible research platform for a wide range of applications based on either Exact Mass UPLC®/API/MS/MS or Exact Mass MALDI/MS/MS.          SYNAPT MS is a next generation platform that can be configured to meet a wide range of complex analytical challenges via:    

    
• Next generation tandem mass spectrometry platform– fully upgradeable on site to incorporate SYNAPT HDMS™ functionality, extending your capabilities to make new discoveries possible.    
• UPLC/MSE – Avoids under sampling of complex mixtures for more comprehensive, confident, and rigorous profiling of proteins, metabolites, and impurities.    
• Triwave™ Technology – dual-stage collision cell configuration provides optimized MS/MS for comprehensive structural characterization.    
• Exchangeable vacuum MALDI source – Solid state 200 Hz lazer provides rapid analysis in protein profiling and tissue imaging studies.    
• Optimized precursor ion selection – 4, 8, and 32 kDa quadrupole options provide high selectivity for MS/MS analysis of small molecules to protein complexes.    
• Source options – LockSpray™, NanoLockSpray™, IonSABRE™, APCI, ESCi®, and APPI provide comprehensive detection of chemically diverse compounds.    

     Atmospheric Solids Analysis Probe          The AtmosphericSolids Analysis Probe (ASAP) is a powerful tool for the rapid direct analysis of volatile and semi-volatile, solid and liquid samples using atmospheric pressure ionization.          The ASAP technique utilizes the heated nitrogen desolvation gas to vaporize the sample and a corona discharge for sample ionization. This allows low polarity compounds not amenable to ESI, APcI, and APPI to be ionized with a high degree of sensitivity. Furthermore complex mixtures can be analyzed without the need for any sample preparation.          The AtmosphericSolids Analysis Probe is readily fitted to a standard API source by replacing either the ESI or APcI probe assembly. A corona discharge pin also requires fitting. The close proximity of the sample, when loaded onto a glass capillary tube, to the point of ionization and the MS inlet, improves sensitivity whilst also ensuring safety due to the enclosed source housing.          Instruments are operated in combined Electrospray/APcI mode (ESCi®). This provides for the acquisition of both analyte data in APcI mode and reference data in electrospray mode, enabling exact mass measurements to be produced.     The probe is designed to have both fixed & removable sections to allow rapid sample introduction without removal of the complete assembly ensuring stable source conditions.