Brookhaven Instruments - BI-MwA

Manufactured by  Brookhaven Instruments
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Molecular Weight Analyzer

Quickly, easily, and accurately determine absolute molecular weights of proteins and polymers. Eliminate SEC/GPC column calibration and improve data quality. Rapidly reduce uncertainty with no assumptions required and true extrapolation to zero angle. Best of all, the BI-MwA has the highest performance/price ratio of any light scattering detector used for molecular weight determination.  
  
The BI-MwA is simple to use, but incorporates sophisticated features. Inject your sample into the low-volume, 7-angle flow cell. The sample is illuminated by a temperature stabilized, precision power-controlled diode laser. The ultra-stable, high-sensitivity, low-noise CCD detector automatically collects the scattered light. Then, the software extrapolates the data to zero angle for the absolute molecular weight determination.  
Two Ways to Use the BI-MwA  
  
There are two ways to use the BI-MwA: determine the molecular weight distribution (flow mode); determine the average molecular weight (batch-mode). Macromolecular samples often have a range of molecular weights. In some cases, this distribution is quite narrow, and in other cases, it is very broad and/or multimodal. Variations in the distribution can indicate the presence of impurities or aggregation. However, for other applications, the average molecular weight is sufficient to characterize your sample. Sometimes the distribution is of interest, and sometimes the overall average is sufficient. Either way, the BI-MwA is the right tool for the job.
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Features of BI-MwA

Features Include:  

  • Multiangle, laser light scattering detector/for SEC/GPC or standalone use  
  • Proteins & polymers  
  • Polysaccharides & biopolymers  
  • Study protein aggregation, oligomerization, & complex formation  
  • Mn, Mw, Mz, Rg, A2  
  • Small footprint, stackable  
  • RI and Viscosity detectors optional
General Specifications
Depth 380 mm
Height195 nm
Width 210 mm
Weight5.5 kg
Additional Specifications

Cell/Optics  
Angles: 7, nominally 35, 50, 75, 90, 105, 130, and 145 degrees.  
Fiber: Low numerical aperture, integral to cell.  
Maximum Pressure: 3.5 MPa (500 psi) maximum.  
Fittings: Standard HPLC Inlet/Outlet on front panel.  
Volume: Cell, 100 µL nominal; Scattering, 20 nL nominal.  
Laser: Temp. stabilized w/prec. power control, 35 mW, 635 nm, vertically polarized.  
Cell: PEEK std., options on request.  
Electronics/Detector  
Control: Integrated, dedicated, powerful microcontroller. USB driven.   
Detector: CCD, ultra-high sensitivity and spatial uniformity.  
Analog Inputs: 4 standard, with 16 and 24 bit resolution, 15 additional analog channels (optional) with 24-bit resolution. Computer selectable gain adjustment. Suitable for use with most common RI, UV, viscometer, thermocouple, thermistor, injector, pressure gauge and pump outputs.  
Power Requirements: 100/115/220/240 VAC, 50/60 Hz,25 Watts  
Certificates: CE Certified

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