Molecular Devices - CellKey System
Label-free, real-time analysis of cell surface receptor activity in a single-assay format.
The system's underlying technology is Cellular Dieletric Spectroscopy (CDS) — an impedance based measurement system that eliminates the need for tags, dyes, or specialized reagents. Learn more about CDS.
The system was designed to meet the needs of drug discovery scientists-- from target identification and validation through lead optimization. It is sensitive enough to consistently monitor ligand-mediated activation of endogenous receptors expressed in adherent or suspension cell lines and primary cells. The benefit of this is the ability to generate more physiologically relevant data than obtained when using genetically and chemically manipulated cells.
The CellKey™ platform package is a fully integrated solution comprised of the instrument with on-board temperature control and fluidics, and an extensive software suite to manage the information-rich, real-time kinetic data generated.
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Features of CellKey System
Features and Benefits
Reliable measurement of endogenous receptors: The CellKey™ System sensitivity allows biorelevant measurements of receptor activity in cell lines and primary cells. Label free: Expensive tags, dyes, or specialized reagents are not required. Unique kinetic response data: CellKey™ response profiles directly correlate to receptor mediated signal transduction pathways, thus enabling identification of the underlying mechanism of action of lead compounds. Universal platform: Single platform and assay methodology measures both G protein-coupled receptors (GPCRs) and tyrosine kinase receptors (TKRs). Streamlined assay development: Costly and time consuming biological manipulation of cell lines is avoided. Also, single-assay format can be used for multiple receptor types regardless of signal coupling mechanism. Ease of use: The CellKey™ System is a fully integrated, flexible and intuitive platform.
General Specifications
Depth | 23 in |
Height | 28 in |
Width | 33 in |
Weight | 282 lbs |
Additional Specifications
Measurement Principles: Impedance
Measurement Frequency Range: 1 kHz - 10 MHz
Assay Type: Homogenous, cell-based
Measurement Modes: (Simultaneous fluid addition and read) Endpoint and kinetic measurements
Update Rate: (96-well) 2- or 10-second update rate
Microplate Formats:
CellKey™ Standard 96W microtiter plate: 96-well Sciex proprietary cell plate
Compound plate: 96-well and 384-well compound plates and reservoirs.
Fluidics
Format: 96 well pipettor head
Dispense Speed: Adjustable
Pipettor Height: Adjustable
Compound Mixing Capability: Yes
Dispense Volume: 0 - 200 µL
Temperature Range
Analysis Range: 17-37 °C (Depending on ambient)
Ambient Range: 15-30 °C
Deck Positions
Read positions: One
Compound plate positions: Two
Tip-loading positions: One
Power Supply: 100 - 120 or 200-240V (self adjusting) 50 or 60 Hz, 630 W consumption