Thermo Scientific - UltiMate 3000 RSLCnano System

Manufactured by  Thermo Scientific
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Ultrafast separations, Ultrahigh Resolution, Wide flow-pressure footprint, Continuous direct flow

Optimize your analyses for speed, resolution or sensitivity. The Thermo Scientific™ UltiMate™ 3000 RSLCnano LC system offers better confidence: confidence in performance, confidence in results and confidence in ease of operation. Our 25-year experience in nano LC has resulted in a system that provides better results than ever before, setting a new standard in nano LC applications for Proteomics.
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Features of UltiMate 3000 RSLCnano System

Recommended Use:   

  • For all proteomics labs looking for the highest performance and usability in nano LC/MS.   * It is easily configured as routine nano LC instrument for LC/MS protein identification.   * The versatility and excellent performance allow users to optimize sample throughput and protein identification.   * Routine, advanced and high throughput proteomics applications, like direct-injection, pre-concentration, 2D-LC techniques or tandem operation, are all supported by standardized workflows.   * Customized application setups are easily established for proteomics, metabolomics, intact protein analysis, biopharma and beyond.   * The system’s dual pumps cover a very wide flow range starting at the very low flow rates from 20nL/min. less than 50µm i.d. to 1mm column i.d. flows up to 50µL/min. with the nano pump.   * The loading pump can deliver accurate gradient from 50 to 2500µL/min. for 2D separations or an occasional analytical flow experiment.   

    Exceptional Chromatography Performance:    The UltiMate 3000 RSCLnano system delivers the highest level of performance for your low flow applications:   * Up to three times faster than conventional nano LC systems   * Up to twice the resolution of traditional nano LC systems   * Extensive flow-pressure range for maximum application flexibility   * Continuous direct flow delivery enables uninterrupted, very long ultra-high resolution runs   * Optional stainless-steel-free column flow path protects your analytes from modification and degradation   

    Extended Solution to Match Your Workflows:   * Extensive selection of column formats, traps and chemistries, e.g., 25cm and 50cm long 75µm I.D. Thermo Scientific™ Acclaim™ PepMap™ columns   * Complete MS integration with Thermo Scientific Dionex™ DCMSLink™ software Integrates seamlessly with Thermo Scientific™ EASY-Spray™   * Full range of consumables for all application areas, including nanoViper-based application kits   

    Choose the Right Configuration for You:   * Nano pump, loading pump and heated column compartment integrated in one setup   * Compact separate and heated column compartment featuring a direct nano/cap/micro flow, binary pump and an exceptionally capable loading pump (NCS-3500)   * Optional two-position, six-port or two-position, ten-port Snap-In nano valves (also PEAK versions)   * Four-channel degasser built into the solvent rack (SRD-3400)   * Thermostatted pull-loop well plate autosampler (WPS-3000TPLRS) or thermostatted fractionation sampler for advanced 2D-LC techniques (WPS-3000TFC)   * Variable wavelength UV detector with large choice of low volume U-Z view flow cells (e.g., 3nL)   * nanoViper MS connection kits for Thermo Scientific mass spectrometers   * Optional EASY-Spray source interfaces   * Optional additional nano/cap/micro flow pump module (NCP-3200)   * DCMSLink system control integration software

General Specifications
Injection Volume0.01 to 125 μL
Pump Flow Rate0.02 to 50 µL/min
UV/VIS Noise0.00005 AU at 254 nm
UV/VIS Wavelength190 to 900 nm
Additional Specifications

Flow Rate Ranges: HPG Nano Pump: 20 nL/min–50 ΜL/min (using dedicated flow selectors)    
Flow Selectors (recommended [settable]):    
Nano: 50 nL/min–1000 nL/min    
[1 nL/min–1500 nL/min]    
Capillary: 500 nL/min–10 ΜL/min    
[1 nL/min–15 ΜL/min]    
Micro: 2.5 ΜL/min–50 ΜL/min    
[1 nL/min–50 ΜL/min]    
Custom flow selectors are available on request.    
LPG Micro Pump: 10–2500 ΜL/min (NCS-3500RS only)    
Pressure Range:    
20–800 bar (300–11,600 psi)    
20–500 bar (300–7250 psi), loading pump    
Number of Solvent Channels:    
HPG Nano Pump: 2    
LPG Micro Pump: 3    
Flow Calibration: Semi-automated    
Proportioning Accuracy: 0.9995 from 100–500 nL injections    
Carryover: <0.02% with needle wash (caffeine)    
Optional Sample Cooling: 4 °C–45 °C, at least 22 °C below ambient    
Fraction Collection: Micro fraction collection option [up to 345 bar (5000 psi)]    
Wetted Parts: PEEK, Stainless steel, PCTFE, fused silica    
Dimensions (h × w × d): 16 × 16.5 × 20 in.    
Weight: 22.7 kg (50 lb) including cooling    
Power Requirements: 85–260 V, 50/60 Hz, max. 320 W    
PC Connection: USB; USB hub with three integrated sockets    
I/O Interfaces: Four digital inputs and four programmable outputs    
Data Collection Rate: Up to 200 Hz (in single wavelength mode)    
Maximum Number of Channels: Four    
Drift: 4.0 mAU/h    
Wavelength Range: 190–900 nm    
Noise: Typically <0.05 mAU at 254 nm    
Lamp: Deuterium lamp, Tungsten lamp    
Flow Cell Volume: 3 nL for nano LC; 45 nL for capillary LC; 180 nL for micro LC    
Dimensions (h × w × d): 16 × 42 × 51 cm (6.3 × 16.5 × 20 in.)    
Weight: 15 kg (33 lb)    
Power Requirements: 85–260 V, 50/60 Hz, max. 150 W    
PC Connection: USB    
I/O Interfaces:    
Four digital inputs and four digital outputs    
Two analog inputs (optional DAC module)

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