Bio-Rad Laboratories, Inc. - MyiQ2
Manufactured by Bio-Rad Laboratories, Inc.
Two-Color Real-Time PCR Detection System
MyiQ™2 Two-Color Real-Time PCR Detection System
The MyiQ2 two-color real-time PCR detection system offers two-target analysis capabilities for multiplex PCR and routine detection of single fluorophore experiments using SYBR Green I or other green fluorescent dyes. The system is built on the quality gradient-enabled iCycler thermal cycler and can accurately quantitate targets over a dynamic range of nine orders of magnitude. The iQ5 optical system software, version 2.1, used to control the MyiQ2 system, has advanced features for streamlined gene expression analysis, quantitative assays with multiple standard curves, and other qualitative assays, including allelic discrimination and screening for known mutations.
The MyiQ2 two-color real-time PCR detection system offers two-target analysis capabilities for multiplex PCR and routine detection of single fluorophore experiments using SYBR Green I or other green fluorescent dyes. The system is built on the quality gradient-enabled iCycler thermal cycler and can accurately quantitate targets over a dynamic range of nine orders of magnitude. The iQ5 optical system software, version 2.1, used to control the MyiQ2 system, has advanced features for streamlined gene expression analysis, quantitative assays with multiple standard curves, and other qualitative assays, including allelic discrimination and screening for known mutations.
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Features of MyiQ2
- Multiplexing of up to 2 fluorophores in each reaction well, enabling 2-target detection
- Advanced gene expression analysis by relative quantity (?CT) or normalized expression (??CT), accounting for differences in reaction efficiency or use of multiple reference genes
- Gene study feature enables direct comparison of gene expression results from up to 5,000 CT data points and multiple plates
- Multiple data reporting options to generate publication-quality statistics and graphs, quickly create customizable reports, or export data from tables with 1-step Export to Excel
- User preference settings for managing data files, analysis settings, and access privileges
General Specifications
Depth | 58 cm |
Height | 39 cm |
Real-Time PCR | 1 |
Width | 29 cm |
Fluor. Excitation Range | 470 to 495 nm |
Gradient Accuracy | 0.4 °C of programmed target at end rows, NIST-traceable |
Weight | 17.6 kg |
Gradient Range | 40 to 100 °C |
Temp. Differential Range | 1 to 25 °C |
Additional Specifications
Base thermal cycler: iCycler
Sample capacity: 96 x 0.2 ml tubes or 1 96-well PCR plate
Sample Volume: 10–100 µl
Fluorescence excitation range: Channel 2: 515–545 nm
Fluorescence detection range:
Channel 1: 515–545 nm
Channel 2: 565–585 nm
Linear dynamic range of copy number: Up to 9 orders of magnitude
Row/column uniformity: ± 0.4°C, well-to-well (within row) within 30 sec of reaching target temperature