Eksigent - nanoLC-Ultra system
Manufactured by Eksigent
Introducing the NanoLC-Ultra family of high pressure HPLCs that delivers superior gradient precision at pressures up to 10,000 psi.
The next generation NanoLC system from Eksigent promises to deliver high resolution separations, combined with excellent reproducibility, resulting in improved identification of low abundance peptides/proteins with nanoLC-MS.
The higher pressure capability of the NanoLC-Ultra platform allows the use of longer columns and/or columns packed with smaller particles for higher resolution separations of complex (digested) protein samples. Moreover, the NanoLC-Ultra family features self-priming and self-purging pumps for easier changing of solvents, and a temperature-controlled column compartment for maintaining exceptional retention time reproducibility.
Eksigent’s proven Microfluidic Flow Control™ (MFC) technology guarantees reliable and reproducible results, while integration with major mass spectrometer software packages makes the system a seamless addition to almost any nanoLC-MS workflow.
A range of different NanoLC-Ultra system configurations are available for various proteomics applications—from single gradient systems for direct sample injection, to dual gradient systems for multidimensional separations, and dual column set-up for increased throughput.
The higher pressure capability of the NanoLC-Ultra platform allows the use of longer columns and/or columns packed with smaller particles for higher resolution separations of complex (digested) protein samples. Moreover, the NanoLC-Ultra family features self-priming and self-purging pumps for easier changing of solvents, and a temperature-controlled column compartment for maintaining exceptional retention time reproducibility.
Eksigent’s proven Microfluidic Flow Control™ (MFC) technology guarantees reliable and reproducible results, while integration with major mass spectrometer software packages makes the system a seamless addition to almost any nanoLC-MS workflow.
A range of different NanoLC-Ultra system configurations are available for various proteomics applications—from single gradient systems for direct sample injection, to dual gradient systems for multidimensional separations, and dual column set-up for increased throughput.
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Features of nanoLC-Ultra system
- Splitless, nano-scale flow control
- 10,000 psi maximum pressure
- Retention time reproducibility
- Peak parking
- Mass spec software integration
General Specifications
Depth | 24 in |
Height | 18 in |
Maximum Pressure | 10000 psi |
Optional Flow Rate | 1 to 10 µL/min |
Width | 14 in |
Power Requirements | 100-240 V AC |
Peak Power | 400 VA |
Standard Flow Rate | 1 to 30 µL/min |
Weight | 70 lbs |
Additional Specifications
Working temperature: 15º to 30ºC
Standard flow rate ranges:
- Loading pump: 1-30 µl/min
- Nano gradient: 50-500 nl/min (up to 1000 nl/min at reduced maximum pressure)
- Micro gradient: 1-10 µl/min (up to 20 ul/min at reduced maximum pressure)
Flow rate accuracy: <1%
Flow rate precision: <0.5% @ 200 nl/min
Gradient accuracy: <1% @ 200 nl/min
Gradient delay: <25 nl
Column oven: ambient + 5ºC to 40ºC (±1 ºC)
Built-in 10-port valve:
- 1/32” connection; 0.1 mm bore; port-to-port
- Volume < 25 nl; max. pressure 10,000 psi
- Stainless Steel with proprietary coating on valve stator
Wetted parts: Stainless Steel, Titanium, PEEK, Fused Silica, FEP, proprietary inert coating
I/O:
- 1 x USB for instrument control
- 2 x RS232 serial output
Each pump channel:
Instrument control: Eksigent software with plug-ins for Thermo Electron’s Xcalibur®, Bruker Daltonics’ HyStar® and ABI/Sciex Analyst® software packages