SCIEX - cHiPLC™-nanoflex
For research use only. Not for use in diagnostic procedures.
The cHiPLC® system is a chip-based platform that, in combination with the nanoLC 400 system, delivers superior sensitivity, column-to-column reproducibility, and exceptional ease of operation.
The cHiPLC system is a "docking station" for up to three microfluidic chips. The system's flexible design and built-in 10-port nano valve allow for easy switching between different types of experiments such as direct injection and trap-loading.
Chips containing a nanoLC column, microLC column, or trap column can be exchanged in seconds. A perfect dead-volume-free connection is made every time as the cHiPLC column or trap column is automatically aligned with a special connector chip.
All alignment is achieved through the use of highly reproducible microfabrication techniques similar to those used in the microelectronics industry. Additionally, these techniques are used to define fluidic paths and to create a microfabricated weir structure for stationary phase particle retention. Chips are temperature controlled to guarantee reproducible retention times and improved separations.
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Features of cHiPLC™-nanoflex
Column design
Special care has been given to the design of both the trap-chips and analytical column-chips in order to achieve separations that are equal or better than separations obtained using packed capillaries (see Figure 1). The use of fused silica allows for cylindrical channels for packing nanoLC columns and traps. Instead of conventional frits made out of fused stationary phase particles, our cHiPLC columns use a unique weir structure to retain the stationary phase particles in the column. These weirs are more reproducible to fabricate, while their dead-volume is virtually zero (~13 pL). In addition, the adsorption of sample components that can occur with frit material is not an issue with these types of structures.
Patented connection system
Connections to and from each chip are made using a patented connection system that can connect up to seven channels to the outside world with a dead volume of less than 1 nl. The force used to connect the chip is pre-set, so that every time the user exchanges a chip, a leak-free connection is obtained without any required user adjustments.
Increased column-to-column reproducibility
Besides the ease of replacing a nanoLC column or trap in seconds, the use of our cHiPLC columns also increases column-to-column reproducibility. All chips are exactly identical, and our packing procedure guarantees the best possible column-to-column reproducibility in nanoLC (see Figure 2). This is of importance for applications where retention time stability over longer periods of time and over multiple columns is important. Examples are the use of retention time in combination with accurate mass in peptide/protein identification and scheduling MRMs for peptide quantitation in biomarker validation.
Simple operation
The cHiPLC-nanoflex allows for easy switching between direct injection, trap-loading and dual column with direct injection experiments. This is achieved through the use of a fluidic jumper chip, which routes fluid appropriately for the desired experiment. This jumper chip is as easily changed as the column and trap chips.
Nano-spray source compatibility
In addition, the cHiPLC-nanoflex can be used in combination with all Eksigent nanoLC systems along with any mass spectrometer/nanospray source. The cHiPLC-nanoflex allows for easy switching between direct injection, trap-loading and dual column with direct injection experiments. This is achieved through the use of a fluidic jumper chip, which routes fluid appropriately for the desired experiment. This jumper chip is as easily changed as the column and trap chips.
Nano-spray source compatibility
In addition, the cHiPLC-nanoflex can be used in combination with all Eksigent nanoLC systems along with any mass spectrometer/nanospray source.
General Specifications
| Depth | 10 in |
| Height | 10.5 in |
| Maximum Pressure | 4000 psi |
| Width | 6.5 in |
| Power Requirements | 100-240 VAC, 50/60 Hz, 2.5 A |
| Weight | 10 lbs |
Additional Specifications
Working temperature: 15 to 30 ºC
Temperature control: 25 to 60 ºC +/- 0.1 ºC
Maximum pressure: 4000 psi
Valve:
[list]
[*] PAEK 10 port valve
[*] 1/32 connections; 100 µm bore
[*] Port-to-port volume < 25 nl
[*] Max. pressure 5,000 psi
[/list]
Wetted parts: PEEK, PAEK, Valcon E, fused silica, tefzel
Column dimensions: 75 µm ID x 15 cm
Trap column dimensions: 200 µm ID x 0.5 mm
Instrument control: All temperature control is achieved through the LCD interface on the nanoflex; valve control is achieved through the Eksigent software which controls the pump system. Valve control can be disabled at the nanoflex through the LCD interface.