Wyatt Technology - Eclipse 3+
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The Eclipse™ 3+ represents Wyatt Technology's fourth generation AF4 system and is the market-leading se...
The Eclipse™ 3+ represents Wyatt Technology's fourth generation AF4 system and is the market-leading separation system using the Asymmetric-Flow Field-Flow Fractionation (AF4 or FFF) technique. The Eclipse™ 3 integrates easily with Wyatt's Multi-Angle Light Scattering (MALS) instruments: DAWN®, miniDAWN™, as well as the Optilab™ rEX. The combined system provides absolute size and molar mass distributions of the particles/molecules separated by the AF4 unit. The combined system solves the most difficult sizing tasks in the macromolecular and colloidal arenas. Its resolution is similar to sedimentation equilibrium, but it surpasses even this benchmark-sizing tool when one considers its flexibility, speed, and ease of use.
Typical Applications:
The Eclipse™ 3+ is invaluable and indispensable in key areas of research and development in molecular biology, nanotechnology, and environmental analyses, including the separation and characterization of proteins and aggregates, liposomes, emulsions, viral particles, polysaccharides, nanoparticles, polymer latex particles, colloidal soil suspensions, humic substances, chemical mechanical polishing slurries (CMP), and more.
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Features of Eclipse 3+
Advantages of the Asymmetric-Flow Field-Flow-Fractionation (AF4) Technique:
- Separates both soluble and colloidal components over a wide size range; especially unmatched powers in sub-micrometer range
- Large dynamic range: from a few nanometers to a few micrometers
- Rapid analysis: typically 10 to 30 minutes
- Absence of shearing forces (no stationary phase; separation takes part in separation channel)
- Ultra-high resolution separations, comparable to ultra centrifugation
- Simplifies analysis by eliminating many time-consuming sample preparation steps
- Adjustable and flexible separation ability: very efficient for complex samples
- Direct injection: minimal sample preparation necessary
- Interfaces with modern analytical methods like MALS, MALDI-TOF, etc.
- Fractions can be collected and used for off-line analysis (with electron microscopy, MS, ELISA etc.)
The Eclipse™ 3+ is the world's most advanced AF4 system!
- Fully automated, software controlled system with only one pump to generate detector flow, cross flow and injection flow. This innovative concept to generate all three flow streams from one main flow has been a breakthrough for the AF4 method!
- One chassis contains all necessary electronic and fluid components, designed to stack with Wyatt Technology detectors (DAWN, Optilab, ViscoStar)
- Eclipse Chassis can be ordered in three versions: for aqueous solvents (biocompatible), for organic solvents and in a metal-free version
- The Eclipse integrates into various market-leading HPLC packages, such as Agilent and Shimadzu.
- Independent, continuous measurement of the cross-flow and injection flow rate, which allows software controlled check on the system performance
- Motor driven, software controlled needle valves for focus balance and injection flow rate control
- Proprietary pressure sensor without dead-volume provides continuous read-out of channel pressure
- High performance electronic controller inside the chassis (PLC- programmable logic controller) with TCP/IP communication to PC
- LCD-Display with system status information
- CE classification surpasses laboratory standards for safety and emission and reaches industry grade
General Specifications
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