The Calypso® II interfaces with a Wyatt multi-angle light-scattering detector (DAWN® HELEOS® II or miniDAWN™ TREOS®) in order to analyze biomolecular interactions, without labeling or immobilization, to determine affinity, true molecular stoichiometry, and the kinetics of self-assembly, aggregation or dissociation of macromolecular complexes, by means of composition-gradient multi-angle light scattering (CG-MALS). A typical system might include a concentration detector such as Wyatt's Optilab® T-rEX™ or a 3rd-party on-line UV absorption detector.      
     
The CALYPSO™ software for control, data acquisition and analysis offers a comprehensive suite of association models for studying self-association and hetero-association including high-order oligomerization, simultaneous self- and hetero-association, and multi-valent interactions. This unique technology nicely complements surface plasmon resonance (SPR), isothermal titration calorimetry (ITC), and other biophysical techniques for characterizing interactions; in addition, it is much faster and simpler than analytical ultracentrifugation.