Discussion Category: Microplate Readers
Berthold lumat LB 9507
I have the alert mYCOALERT with luciferase assayfirefly substrate(kitPromega E1910). What does it mean?
I have test the linearity of the measure :
sample undiluted : 1479481 dil 1/2 :802400 dil 1/4 351486
dil 1/8 156044 1/16 88622. Are they good values. Please could you betweengive me the range of measure of this instrument ( measuring range).If the measure is too high, with what can I dilute the sample?
Yours faithfully
josiane Denis
Reply byBernd Hutter
Hi Josiane,
what you did is ideal to judge the linearity and the dynamic range. plotting your data gives an R² of 0.9966 which is perfect. Your samples are all within the range of the instruments
bernd
Bernd Hutter