Discussion Category:  HPLC Columns

Yarra SEC-3000 compatibility with methanol


I have a 'Yarra 3000 SEC'. It was working very fine, giving nice shape peaks. I left it in 100% methanol overnight. In the morning, I ran my buffer and started running samples, it is giving broad peaks.

I was trying to find the compatibility of mentioned column with 100% methanol. I was under the impression that silica columns can be washed with 100% methanol.

Please send your views.

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