Discussion Category: Electroporation
My axopatch 200B is registering an overload as soon as the pipette is put into the bath. Normally this means a grounding issue however I have removed all non-essential equipment from the rig and have ensured everything remaining is all grounded into the back of the axopatch itself and the overload is still present. It is not present in Current clamp mode.
I have tested the axopatch according to the axon guide book and it is behaving normally which indicates to me there is not an issue with the amplifer itself, however I can see no issues with grounding that could be causing this problem so I am hoping you have some suggestions as to what could be the cause of the overload.
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